Construction Of Complex Microbial System ZFX-1 Decomposing The Corn Stalks And Initial Exploration Of Degradation Effect

China is a conventional agriculture country,only corn stalk is more than 200 million tons.every year,But now in the rural areas,soil fertility descline in fields is a widespread,it has seriously restricted the development of modern agriculture.Returning straw is a direct and effective measures,which can resolve this dilemma.The growth of after-reap crop was effected because of the slow decay process and bad effect returning straw which limited the application of the technology quitely.Therefore,studies on thedegradation?microbial?inoculun?become more?popular.5 cm humus subsurface soil from woods,rice field and corn field is used as the strains resources in this experiment.Through liquid fermentation test,strains degradation ability of corn straw was studied,and complex strains ZFX-1 components was determined by means of antagonism experiments,and by testing cellulose enzyme and reducing sugar production in solid fermentation experiment,the superiority strains of strains was defined.In addition,by simulating the field test,the effect of complex strains was evaluated.The main results of paper were as follows:Preliminary screening was carried out by Congo red staining.Secondary screening was conducted by paper disintegration method.With corn stalk powder as the only carbon source,cellulose enzyme was detected and degradation rate of corn stalk was studied through liquid fermentation.Eventually,9 corn stalk degradation strains were got,Fungi Z2-6,Z2-7,Z3-4,Z3-5 belong to Fusarium oxysporum and ZOR8 belongs to Trichoderma viride,Actinomycetes F5-1 and F3-1 belong to Streptococcus,bacteria X0-0 and X2-3 belong to Alcaligenes sp and Serratia odorifera respectively.The Z2-6 cellulose enzyme is in the highest,reached up to 190.19 U/ml,the test results also show that its degradation effect is the best,because its corn stalk weightlessness rate was 59.4% after 15 d iquid fermentation.Through the study on growth characteristics of nine cellulose degradation strains,find that their optimum temperature is at 30 ?,the best survival pH ranges between 7.5~8.5.Under the optimal growth condition,fungi ZOR8 cellulose enzyme activity is highest,reached up to 270 U/ml.The Z2-7 can grow under the condition of 4~20 ?,has low temperature resistant characteristics.We found there is no antagonism effect between nine strains through antagonism experiments,all can be used for construction of complex strains.By solid-state fermentation experiment results show that the fungus Z2-6 strains and ZOR8 were detfined as superiority strains of strains,and bacterias have promoting effect to the stability of the system function.Experimental results show that the more abundant complex strains are,the higher cellulose enzyme activity and the reducing sugar production are,thus promoting the degradation of corn straw fibre.9 test strains are used to construct the complex strains,named ZFX-1,Its composition are: the FungusZ2-6.,Z2-7,Z3-4,Z3-5,F5,ZOR8,Actinomycetes F3-1,F5-1,bacteria X0-0 and X2-3.Pot experiment is applied to simulate the field test,results that show the complex strains ZFX-1 have stronger ability of corn stalk than all fungi combination and combination of bacteria and fungi,and ZFX-1 degradation rate of corn stalk is 63.92% after 60 d,that increases by 27% compared with the blank test.