| Contamination of petroleum and its products is an important environmental issue,which needs to be solved urgently.Flower plants as environmentally friendly repair due to its beautiful and no secondary pollution and widespread concern,but the plant’s tolerance to oil pollution is to play its prerequisite for repair capacity.Based on previous studies,a significant up-regulation of the β-mannanase gene was established by transcriptome data analysis.The expression of β-mannanase gene was confirmed by the expression of the protein mechanism.In this study,the semi-quantitative and real-time quantitative analysis of the gene under oil stress showed that under the oil stress,the β-mannanase gene was significantly up-regulated at the time of 12-36h.(P = 0.05,p = 0.004)was significantly different from that in the control group(P<0.05),and the significant difference was 0.004 at 36 h and reached the maximum at 36 h.The gene expression at 5-10 g/kg(P = 0.05,p = 0.001).Therefore,the optimal response time of oil treatment in the contaminated soil was 36 h and the concentration was 5-10 g/kg,the gene responds to high concentrations of petroleum contaminated soils earlier than low concentrations of petroleum contaminated soil。Bioinformatics predicts that the gene is localized in the cell wall of phytoremediation-related cells and is transformed with subcellular localization of Allium cepa epidermal cells by gene guns.The function of this gene is related to the cell wall.In order to further explore the response mechanism of the gene to petroleum contaminated soil,two groups of control treatments were set up for the growth of Mirabilis jalapa L.in petroleum contaminated soil,The control group was fed with the hydrolyzate of the gene-mannan oligosaccharide(0.4 mg mL-1),the control group was irrigated with tap water,The activities of enzyme activity and membrane damage were measured under the two groups,① the antioxidant enzyme activity and membrane lipid damage index,the oil pollution concentration of 20 g/kg when the root is still showing a certain tolerance,the leaves in the less than 10 g/kg of oil pollution in the soil have a certain Tolerance,root and leaf in the oil pollution of soil tolerance in a certain difference,the root than the leaves have greater tolerance;② The POD activity increased by 9.5 times at 30 g/kg,and the POD activity increased by 2.57 times at 10 g/kg in 12 h of Mirabilis jalapa L.SOD and CAT were also increased,and mannose oligosaccharides increased the antioxidant enzyme activity of plants.The malondialdehyde(MDA)in roots decreased by 53%at 36 h and 20g/kg,Treatment time and oil pollution conditions,the content of malondialdehyde also reduced to varying degrees,plant resistance to enhanced.The antioxidant enzyme system of Mirabilis jalapa L.effectively protected the changes of plant,oxidase and membrane damage index of Mirabilis jalapa L.under oil pollution stress.The results showed that under the control of oligosaccharide,the activity of antioxidant enzyme、Mirabilis jalapa L.endoside β-mannanase(edMAN activity)and membrane damage were significantly different compared with the control group,but only Membrane damage index and edMAN activity were significantly correlated with mannose oligosaccharides as a whole,Significant and correlations were not synchronized with antioxidant enzymes on the whole,Therefore,the response mechanism of the gene to oil contaminated soil has two main aspects,On the one hand,the gene changes the cell wall structure of the Mirabilis japonicum to improve plant resistance;On the other hand,the gene-related mannose oligosaccharides as an inducing substance,through the induction of resistance,Antioxidant enzyme activity increased,It is helpful for the improvement of soil resistance to the oil contaminated soil of Mirabilis jalapa L.. |
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