The Construction Of Responsive Magnetocaloric Drug Delivery Systems And Their Application Of Cell Imaging

With the development of science and medical technology,some scientists pay attention to the treatment and diaglosis of cancer,which could not be mastered completely.Therefore,many kinds of anticancer drug have been developed in succession.The clinical assays indicate that some problems which are based on anticancer drug need to be solved.It could be endocytosed by normal cells nonspecifically,destroy the process of cell proliferation and so on.Therefore,the article mainly designs and constructs some responsive drug delivery systems for cancer,which could reduce the damage of normal cell in the future treatment.We mainly designed four responsive drug delivery systems:1.We designed a temperature and pH dual responsive drug delivery system Fe3O4/PNIPAM/5-Fu@mSiO2-ZnO in this section.The thermo-responsive drug delivery process is achieved through the magnetocaloric property of Fe3O4 and the lowest critical solution temperature?LCST?of thermo-sensitive polymer PNIPAM.When the system is close to tumor cell,the pH responsive property of ZnO could make sure that drug was targeted delivery to cancer cells.It avoids the side effects,which is caused by drug leakage.The result indicates that the drug delivery system displays the pH and temperature responsive ability.Besides that,the result of MTT assay shows that the rate of cell viability is 78%for Fe3O4/PNIPAM@DmSiO2-ZnO and that of Fe3O4/PNIPAM/5-Fu@mSiO2-ZnO is only 20%at the same concentration.2.We designed a temperature responsive drug delivery system Fe3O4/PNIPAM/5-Fu@mSiO2-CHI/R6G.,which could be used in cell imaging.The result indicates that temperature of Fe3O4/PNIPAM/5-Fu@mSiO2-CHI/R6G solution?10mg/ml?could raise to 45? in 300 seconds under magnetic field of 37.680e.Therefore,it is potential for magnetocaloric cancer therapy.Besides that,the system expresses the excellent thermo-responsive drug delivery in different temperature?25?and 45?C?.The MTT and cell imaging assays prove that the system has excellent biocompatibility and function of cell imaging.3.A tri-functional drug delivery system Mn02/Fe3O4/5-Fu@CHI/R6G is designed in this work,which is related with hyperthermia therapy,medication and photo dynamic therapy?PDT?.The GSH responsive drug delivery process is realized by the reaction of Mn02 and reduced glutathione?GSH?.It reduced the damage for normal cells largely.The test result indicates that the cumulative quantity of drug delivery under the GSH concentration of 40mM?the characteristic concentration of cancer cell?is more than that of 10 mM?normal cell?.Besides that,under the visible light irradiation,the system could produce singlet oxygen in acidic H₂O₂ solution,which could inhibit tumor cell growth obviously.It plays a coordinated role of cancer treatment.We also do the experiments of MTT and cell imaging.4.Based on the excellent biocompatibility and conductivity,a graphene?GO?based drug delivery system Fe3O4/5-Fu/PNIPAM@mSiO2-GO is synthesized.Under the visible light irradiation,it could produce singlet oxygen with H₂O₂ in an acidic solution.It aims to kill the hypoxic tumor cells.The experiment result indicates that the system Fe3O4/5-Fu/PNIPAM@mSiO2-GO shows the excellent thermo-responsive drug delivery in different temperature?25? and 45??.The thermo-responsive function could reduce the side effect in some degree.The MTT assay demonstrates that the system has excellent biocompatibility and ability of killing tumor cells.