Applications Of Raman Spectroscopy In Ion Detection,Chiral Recognition And Structural Analysis

Raman spectroscopy is an important technique to detect molecules and obtain their structural information.With the rapid development and progress of Raman technique,Raman spectroscopy has played an irreplaceable role in many fields such as scientific research and real applications with its sensitive,convenient,non-destructive and high selectivity.Combimg the Raman spectroscopy and other techniques,such as hydrophobic surface and surface enhancement,the applications of Raman spectroscopy are studied in various fields such as potassium ion detection,chiral recognition and structural analysis.These research opened up the applications of Raman spectroscopy in the actual production.The main research contents are as follows:1)Detection of physiological potassium ions level in human serum by Raman scattering spectroscopyPotassium ion plays an important role in the life system.Potassium ion does not have Raman activity,while it can react with sodium cobaltinitrite of Raman activity.After the reaction with sodium potassium nitrite,the characteristic peak of sodium cobaltinitrite shifted from 837 cm-1 to 827 cm-1.The characteristic reaction was combined with a hydrophobic surface which having a concentration characteristic,and the reacted substance was concentrated on a hydrophobic surface.Based on the Raman spectroscopy technique,the detection of potassium ions was achieved.The method was applied to the serum system to detect the linearity of potassium in serum from 0.10 m M to 50.00 m M.2)Surface-enhanced Raman spectroscopy study on the structure changes of4-Mercaptophenylboronic Acid?4-MPBA?under different p H conditionsThe structure of the 4-MPBA molecule and its adsorption on the surface of the metal substrate are susceptible to the change of the p H value.The SERS spectra are used to study the adsorption of 4-MPBA molecules on the surface of silver nanoparticles with the change of p H.The molecules begin to hydrolyze when the p H reaches 6,and when the p H reaches the molecular p Ka,the molecules are almost completely hydrolyzed.With the deepening of the degree of alkalinity,the molecules gradually form boric acid anhydride adsorption on the surface of silver nanoparticles.This research provides a theoretical foundation for the study based on mercaptobenzene boronic acid.3)Enantioselective Discrimination of Alcohols by Hydrogen BondingThe recognition of chiral enantiomers is of great significance.We proposed an enantiomeric recognition method based on SERS technology and achiral labeling.The Ag-MPBA hybrid system was firstly constructed.The hydrogen bonds were formed between MPBA molecules and chiral enantiomers when Ag-MPBA assemble system locating in chiral enantiomeric environment.Differences emerged in the hydrogen bond formed between MPBA molecules and deverse chiral enantiomers.SERS spectra had unlike relative intensities due to the differences in hydrogen bonds.The discrepancy in SERS spectra can be observed in selected kinds of chiral alcohols.Based on the difference in the SERS spectra of chiral selectors,the diatinction between the two enantiomers can be realized.The method doesn't need chiral reagents or circularly polarized light,which provides a new way for the chiral recognition.4)A Nano-Cu_2–x S Material as a Substrate for TestingThe nano-sized Cu_2–x S materials with infrared and near-infrared regions were prepared and the SERS spectra of the probe molecules were detected in the infrared and near-infrared regions.The material as a substrate provides a new method forobtaining the molecular structure of the material and the intermolecular interaction.