The Screening And Identification Of Bio-control Actinomycetes And The Study Of Its Inhibition To The Pathogen Of Apple Tree Canker

Apple tree valsa canker occurs seriously year by year,and it has become one of the important factors which restrict the rapid development of apple industry.As a traditional method,chemical fungicides have caused a series of problems such as environmental pollution,pesticide residues and health hazards.With the health of people increasing awareness of environmental protection,efficient exploration of Apple tree canker biological control technology has become an important subject to research and solve the production.In view of this,the test of antagonistic inhibition were studied and the mechanism of identification were discussed,researches the resistance of biological control and discusses the influence of Antagonistic Bacteria on post harvest storage quality of apple,the results are as follows:Firstly,Four strains of antagonistic strains against apple tree rot were screened by traditional confrontation culture method screening.The strains named CH10,CH4-A2,Be44-A(1),Be3-B6(A),of which the antagonistic effect of CH10 was the best,and it was identified as Streptomyces by 16 S r DNA and it was selected for further study.Secondly,The inhibition effect of 8 strains of pathogens on fruits and vegetables were inhibited by CH10 strain,and the inhibition effection of Valsa mali Akesu among the pathogens of Akesu apple tree was the best.Thirdly,The treadmill test proved that CH10 fermentation liquid had significant inhibitory effection on Apple tree canker,the results showed that the antagonistic substance had good antibacterial effect on apple tree rotting pathogen Valsa mali Akesu.The antagonistic circle diameter was 3.46 cm,the inhibition rate of 62.50%.Finally,The hardness,solid content,total acid and ascorbic acid were measured after 10 days of harvested storage apple by the strain CH10.The difference between these indexes and the control was not obvious.Fifthly,In addition,in vivo experiments confirmed CH10 can promote CHT,β-1,3-glucanase,PPO were showed first increased and then decreased the trend to improve their disease resistance.