The Design,Synthesis And Application Of Fluorescent Probes For HClO And Hg²⁺

Fluorescence analysis,featured by simple operation and high sensitivity,is widely applied in the fields of medical,life and environmental sciences,which can realize the real-time and dynamic monitoring in biological body.In this thesis,three fluorescent probes have been designed and synthesized for the detection of HClO and Hg²⁺,respectively:1.A new type of “turn-on” Cl O-fluorescent probe PHC2 was designed and synthesized based on 2-（2’-Hydroxylphenyl）Benzothiazole（HBT）.The effect of Excited-state Intramolecular Proton Transfer（ESIPT）of the probe was restored by the oxidation reaction between the azo moiety and HCl O.Also,probe presented high specificity selection and sensitivity.2.2-（2’-Hydroxylphenyl）Benzothiazole（HBT）based “turn-on” fluorescent ClO^-probe PHC3 has been synthesized.The probe features good selectivity and sensitivity to ClO^-under PBS:Et OH（V/V = 2:8,pH 7.4,1% MeCN）conditions.3 A “ratiometric” fluorescent response Hg²⁺ probe PHC4 has been designed and synthesized by combination of coumarin and thioacetal.In the addition of concerntraion of Hg²⁺ in PBS（pH 7.4）,the blue fluorescence emission at 497 nm gradually decreased,while the new yellow fluorescence emission at 566 nm gradually increased.The probe shows good selectivity and excellent sensitivity.Furthermore,the probe could monitor Hg²⁺ in HeLa cells.