Study On Operational Efficiency And Microbial Communities During Different Culture Stage Of The Anaerobic Phosphine Released System

Aiming at the phenomenon that phosphate can be released in the form of gaseous phosphine under anaerobic conditions.In this experiment,the ASBR process was used to investigate the biological phosphorus removal by the generation of phosphine.The 1~#ASBR which was without any flocculant addition and the addition of polyquaternium flocculant to the 2~#ASBR.Examining about phosphorus removal and phosphine production efficiency in the different culture stage.Exploring the sludge particle size and morphological characteristics in the stable period;dynamic monitoring the changes of the organic,phosphate,alkaline phosphatase,dehydrogenase and MBP of the typical cycle in the stable period of the two sets systems.The changes of microbial consortia structure in each culture stage were dynamically tracked and the function of microbial populations to produce phosphine were analyzed,the main results are as follows:After different way of cultivation,the mean particle size of 1~#ASBR is 154.26?m and2~#ASBR is 218.01?m,the specific surface of 2~#is smaller than that of 1~#and the homogeneity of 2~# is bigger than that of 1~#,the voids of 2~# is larger than that of 1~#,which indicates the adsorption capacity of 2~# is better than that of 1~#.The SEM results show that the 1~# sludge is flocculent and the 2~# sludge is small granular,indicating that the regular addition of polyquaternium flocculant can change the sludge morphology.In the 1d~160d culture period,the capacity of organic matter removal was mostly same of the two anaerobic reactors,the removal rate of COD can reach to more than 93.00% when the influent COD concentration was 3.60~3.80g/L,the phosphate removal rates and phosphine production were improved step by step for two systems during 1d~40d,1~#ASBR phosphate removal rate increased from 18.22% to 32.73%,the values of phosphine production increased from 0.04?g/m3 to 1.26?g/m3;2~#ASBR phosphate removal rate increased from 19.43% to 32.10%,the output of phosphine production increased from 0.04?g/m3 to 1.63?g/m3,this period was domestication stage for 1~#ASBR and 2~#ASBR,the microbial community of 1~#ASBR and 2~#ASBR systems were similar,and the dominantbacteria were Methanosaeta,Treponema,Syntrophus and Blvii28.the phosphate removal rates and phosphine production were stable and high for 1~#ASBR during 90d~105d,the average phosphate removal rate was 39.99% and the average phosphine production was 3.26?g/m3,the dominant bacteria were Methanosaeta,Longilinea,E6,T78 and DCE29;2~#ASBR was stable during 70d~105d,the average phosphate removal rate was50.90% and the average phosphine production was 5.08?g/m3.the results showed that the dominant bacteria were Methanobacterium,Methanosaeta,Sedimentibacter,Syntrophus,SHD-231,E6 and T78,The addition of polyquaternium flocculant to anaerobic phosphating process can change the composition of sludge microbial consortia structure and the abundance value,which can improve the efficiency of phosphate removal and phosphine production.the phosphate removal rates and phosphine production were decreased sharply for1~#ASBR and 2~#ASBR during 140d~160d,this period was collapse stage for the two system the removal rate of phosphate were 11.06% and 17.81% respectively at 160 d,the output of phosphine were 1.66?g/m3 and 1.87?g/m3 respectively at 160 d.The main reason for the collapse of the two systems is likely the high temperature outside lead to the excessive emergence of the new dominant species Kosmotoga,abundance value of up to 30% or more,greatly hindered the anaerobic biological phosphorus removal and phosphate production.Macroeconomic control of culture temperature may inhibit the growth of such bacteria.The change of COD,phosphate,dehydrogenase,alkaline phosphatase and MBP production was dynamically monitored every hour in the typical cycle.In both systems,dehydrogenase activity,alkaline phosphatase activity and MBP production overall were increased rapidly and then decreased slowly.The activity of 1~#ASBR phosphatase,dehydrogenase and MBP reached the maximum at the 2nd hour,the value were1.46mg/gTSS·h,51.34mg/gTSS·h and 503.31pg/g dry sludge respectively,the organic matter was basically degraded at 6h,the phosphate effluent concentration was 28.11mg/L;2~#ASBR phosphatase activity and bound phosphine production reached the maximum at 3h,1.70mg/gTSS·h and 993.33pg/g dry sludge respectively.The dehydrogenase activity reached the maximum at the 4th hour,and was 54.23mg/gTSS·h.The degradation of COD was completed at the 5th hour,and the phosphate effluent concentration was 27.50 mg/L,the correlation coefficient(R2)of MBP and activity of alkaline phosphatase and dehydrogenase were 0.82 and 0.68,which show that these two kinds of enzymes play an important role in the formation of phosphine.