| Metal Organic Frameworks?MOFs?has attracted much attention in recent year,because it's high surface area?adjustable pore size?structure and functional diversity.The sensors based on MOFs has been applied in detect biomolecules?inorganic molecules or organic molecules.In this thesis,the solution stable MOFs was used to contrast a biosensor to detect biomolecules and inorganic molecules.Sensors contrasted,could detected target in highly efficient and highly sensitive.The thesis consists of 5 chapters:In chapter 1,a general introduction about MOFs was made,including the basic concept,the classification,development status,etc.Then,their synthesis methods?functionality modification and their applications in the field of biochemistry sensor,separation and catalytic.The purpose and the main contents of this thesis had been pointed out also.In chapter 2,mainly introduced the method to synthesis two kinds of MOFs we needed to contrast biosensors,Fe3O4@g-C3N4@MOF?magnetic gH hybrid composites?and UIO-66-NH2.Additionally,a series of structural characterization of the material are investigated,in order to obtain the possibility of their applications in biosensor.In chapter 3,a fluorescence biosensor to detect thrombin was established based on composites.Materials have strong adsorption capacity for dye-labeled aptamers and completely quench the fluorescence of the dye.In the presence of thrombin,the binding between the dye-labeled aptamer and thrombin will alter the conformation of dye-labeled aptamer to form G-quadruplex.Therefore,the interaction between the dye-labeled aptamer and Magnetic gH hybrid composites would be disturbed.Such interactions will cause the releasing of the dye-labeled aptamer from the Magnetic gH hybrid composites,resulting in restoration of dye fluorescence.The fluorescence intensity of the system has a relationship with the thrombin concentration with the linear range of 1.25?20×10-6 mo1·L-1,the detection limit is 3.72 × 10-7 mol·L-1In chapter 4,Urea sensors based on pH response metal organic frameworks was introduced.Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia which increase the pH of solution.When the pH is higher than 7,UIO-66-NH2 presents much stronger fluorescence that is in close association with pH.Because with the increase of pH,are assumed to arise from deprotonation of amino group of 2-aminoterephthalic acid in UIO-66-NH2,based on this principle urea sensor was contrasted.The fluorescence intensity of the system has a relationship with the urea concentration with the linear range of 0.04?0.8 mg·dL-1,the detection limit is 0.0317 mg·dL-1.While the concentration requirements in GB is 20?30 mg·dL-1.In chapter 5,we introduced a Turn-on fluorescensor to detect Sodium sulfide.In difference pH solution environment UIO-66-NH2 has different fluorescence intensity when extract in 330nm.In low pH,the weak fluorescence emission of UIO-66-NH2 is owing to the conjugated double bond system and the high mobility of the ?-electrons.With increase of pH,are assumed to arise from deprotonation of amino group of 2-aminoterephthalic acid in UIO-66-NH2,which lead to enhance fluorescence intensity.The fluorescence intensity of the system has a relationship with the Sodium sulfide concentration with the linear range of 7.5?125×10-6mol·L-1,the detection limit is 7.1×10-6 mol·L-1. |
PDF Full Text Request