Study On The Wearable Electrochemical Biosensor Electrode Based On Low Dimensional Nanomaterials

With the continuous development of electrochemical biosensors,a lot of excellent electrochemical biosensors have been developed,and used in medical,safety testing,food safety and environmental protection and other fields.Because of the biosensors simple detection process,high accuracy and sensitivity,it has become the focus of various detection applications.However,with the widespread of electrochemical biosensors,the research of the sensor is more concerned with the needs of the user,and the sensor equipment tends to miniaturization and portability.Therefore,in recent years,there have been a new type of sensors,such as printed electrodes and enzyme membrane reactors,which have the advantages of high efficiency and stability,and is convenient for users to use and measure.In this paper,a flexible glucose and lactic acid sensor was prepared,and the excellent performance of the enzyme membrane sensor was prepared to achieve the purpose of high efficiency,portability and miniaturization of the sensor.First,conductive polymer films were prepared by adding PEDOT:PSS aqueous solution and MWNT-OH into SBS thin films,and as a sensor electrode,chitosan and glutaraldehyde were used to fix the oxidase to prepare the flexible glucose biosensor and the flexible biosensor with good electrochemical properties.The flexible glucose biosensor was prepared,the linear detection range was 0~6 mM,the linear regression equation was I=2.21*c+1.4455,R~2=0.996,the detection sensitivity was 2.21 ?A/mM,the detection limit of 12 ?M(S/N=3).The flexible lactate biosensor was prepared,the linear detection range was 0.2~1.6 mM,the linear regression equation was I =7.00*c+0.5796,R~2=0.9935,the detection sensitivity was 7.00 ?A/mM,the detection limit of 7.87 ?M(S/N=3).These two biosensors have good stability and anti-jamming performance.Second,graphene oxide was successfully prepared by the improved Hummers method,and G-PtNPs was prepared by solvothermal method,and as a sensitive material to prepare the lactic acid biosensor,with has good electrochemical detection stability and anti-jamming performance,the linear detection range was 0~1.2 mM,the linear regression equation was I =2.08*c+0.1623,R~2=0.990,the detection sensitivity was 2.08 ?A/mM,the detection limit of 12.5 M(S/N=3).At the same time,the flexible lactic acid biosensor,had good stability and reproducibility,and the detection sensitivity of 2.08 ?A/mM,through the real-time detection of human sweat,found that the flexible lactate sensor can be used to detect human sweat.Third,the conductive films was prepared by adding MWNT-OH to the PVB films,and the enzyme was modified to prepare the glucose and lactic acid biosensor with excellent performance.The glucose sensor was prepared using 1% PVB,the linear detection range was 0~3 mM,the linear regression equation was I =0.03*c+0.0305,R~2=0.993,the detection sensitivity was 0.03 ?A/mM,the detection limit of 49.1 ?M(S/N=3).After joining TTF,the linear detection range increased to 0~32 m M,the linear regression equation was I =0.26*c+0.4899,R~2=0.990,the detection sensitivity was 0.26 ?A/mM,the detection limit of 62.8 ?M(S/N=3),the linear detection range greatly improved.In addition,the lactic acid sensor was prepared using 1% PVB,the linear detection range was 0.5~1.5 mM,the linear regression equation was I =0.20*c+0.0967,R~2=0.992,the detection sensitivity was 0.20 ?A/mM,the detection limit of 18.8 ?M(S/N=3).After adding TTF,0.1% GA was used to fixed LOx to prepare the lactate sensor,the linear detection range was 2~5 mM,the linear regression equation was I =1.51*c+1.4956,R~2=0.991,the detection sensitivity was 1.51 ?A/mM,the detection limit of 2.98 ?M(S/N=3),the linear detection range increased.