Study On Preparation And Properties Of Bletilla Striata Polysaccharide Superporous Hydrogel

Bletilla striata polysaccharide(BSP)is also known as Bletilla stripe polysaccharide,Bletilla glucomanan.BSP’s composition is similar to konjac glucomannanare with a backbone of β-glucosidic bond linked mannose and glucose in a molar ratio of 4: 1,which has very good hydrophilicity,and contains a large number of hydroxyl which can be chemically modified.These characteristics suggest that BSP can be used in the preparation of hydrogels.BSP has biological activities including antioxidant activity.It can be used as a hemostatic or anticancer drug for clinical and as an additive or ingredient in the food industry.In order to improve the performance of BSP and broaden its application in the field of medicine,BSP were added to superorous hydrogel(SPH)forming semiinterpenetrating polymer network,which were prepared by foam polymerization method under the action of acrylamide(AM),acrylic acid(AA),N,N’-methylene-bisacrylamide(Bis),ammonium persulfate/N,N,N’,N’-tetramethylethylenediamine(APS/TEMED),NaHCO3 and Foam Stabilizer [poloxamer 407(PF127): poloxamer 188(PF68)= 3: 1(W/W)].The preparation conditions of BSP-SPH were optimized by single factor experiment with the swell ratio as the index.The structural changes were observed by fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy,and the morphological changes were investigated by scanning electron microscopy.Otherwise,the swelling properties and dissolution properties of the gel were studied in different medium solutions.Experimental results:(1)The results showed that BSP-SPH were porous and contained a large number of interconnected pores,and BSP were distributed at the edge of the pores.(2)BSP-SPH can be endowed with good swelling and slow erosion performance by the addition of BSP.The swelling ratio is 200 times.BSP-SPH could levitate in the aqueous medium after water-absorbing swelling due to its low density.The average angle of repose was 31.9 ± 1.6° indicted better fluidity.It was cited wet easily,so store it in airtight container.The swelling ratios of BSP-SPH and SPH in different media were distilled water> pH 7.4 PBS> 0.1 mol/L HCl.The dissolution rate of BSP-SPH in distilled water and different concentrations of NaCl and glucose were lower compared with SPH,and the order of dissolution rate of BSP-SPH were pH 7.4 PBS <0.1 mol/L HCl < pH 6.8PBS in different pH solutions(3)Puerarin dispersible tablets were prepared by wet granulation including the excipients of MCC,starch,dextrin and PVPK30,which were evaluated by dispersible uniform,Single factor experiment to optimize the prescription.The dissolution ratio in vitro of the dispersible tablets were investigated by Ultraviolet spectrophotometer(UV).The dispersible tablets disintegrated within 3 min and the dispersible uniform was better.The cumulative dissolution of dispersible tablets reached 97.2% within 10 min.The influence of the BSP modified material on the tablet disintegration and dissolution performance were preliminary evaluated.(4)The biosafety of BSP-SPH was determined by MTT assay.When the concentration of BSP-SPH extract was 10 mg/mL,there was no significant effect on cell viability(p<0.05).The results showed that BSP-SPH was good biocompatibility.Conclusion:In this study,the preparation process of BSP-SPH was determined.The swelling and dissolution properties of BSP-SPH were improved.It could be used as disintegrant and hydrophilic gel skeleton material.The dispersible tablet of Puerarin was prepared with the BSP-SPH as disintegrant,and the dispersion uniformity and dissolution in vitro were in line with the requirements.All the results shown that BSP-SPH has no obvious cytotoxicity and was good biocompatibility.