Study Of The Colloidal Gold-Basic Immunochromatographic Assay For The Detection Of Nitrofurans

The colloidal gold immunochromatographic rapid test strip for rapid detection of furazolidone metabolite(AOZ),furantoin metabolite(AHD),nitrofurazone metabolite(SEM)and furaltadone metabolite(AMOZ)were developed in this work.For the more,we apply these methods for the visual determination of nitrofuran metabolites in animal-derived food.The diameter of colloidal gold particles was 20 nm.The pH of colloidal gold solution was adjusted 9 and the optimal amount of antibody(2mg/mL)was 10 pL.The dilution of gold labeled antibody was 1:8.The diluted solution of secondary antibody and coating antigen were PB,and dilution were 1:10 and 1:5.Millipore HF90s models of nitrocellulose membrane(NC membrane)don’t need to be blocked.The quantity of secondary antibody and coating antigen is 0.3 μL/cm.The whole detection time was 10 minutes.The limit of detection of colloidal gold immunochromatographic assay for the detection of NPAOZ was 0.5pμ/L.The limit of detection was 0.1μg/kg for detecting AOZ in animal-derived food samples.The diameter of colloidal gold particles was 20 nm.The pH of colloidal gold solution was adjusted 9 and the optimal amount of antibody(2mg/mL)was 10 μL.The dilution of gold labeled antibody was 1:5.The diluted solution of secondary antibody and coating antigen were PB,and dilution were 1:10 and 1:1.Millipore HF90s models of nitrocellulose membrane(NC membrane)don’t need to be blocked.The quantity of secondary antibody and coating antigen is 0.3uL/cm.The whole detection time was 10 minutes.The limit of detection of colloidal gold immunochromatographic assay for the detection of NPAMOZ was 2 pg/L.The limit of detection was 0.5 μg/kg for detecting AMOZ in animal-derived food samples.The diameter of colloidal gold particles was 20 nm.The pH of colloidal gold solution was adjusted 9 and the optimal amount of antibody(2mg/mL)was 20 μL.The dilution of gold labeled antibody was 1:6.The diluted solution of secondary antibody and coating antigen were PB,and dilution were 1:10 and 1:1.Millipore HF90s models of nitrocellulose membrane(NC membrane)don’t need to be blocked.The quantity of secondary antibody and coating antigen is 0.3 uL/cm.Detection time was 10 minutes.The limit of detection of colloidal gold immunochromatographic assay for the detection of NPSEM was 3 μg/L.The limit of detection was 0.5 μg/kg for detecting SEM in animal-derived food samples.The diameter of colloidal gold particles was 20 nm.The pH of colloidal gold solution was adjusted 9 and the optimal amount of antibody(2mg/mL)was 15 μL.The dilution of gold labeled antibody was 1:5.The diluted solution of secondary antibody and coating antigen were PB,and dilution were 1:10 and 1:1.Millipore HF90s models of nitrocellulose membrane(NC membrane)don’t need to be blocked.The quantity of secondary antibody and coating antigen is 0.3 uL/cm.Detection time was 10 minutes.The limit of detection of colloidal gold immunochromatographic assay for the detection of NPAHD was 5 μg/L.The limit of detection was 0.8μpg/kg for detecting AHD in samples.The optimal conditions of colloidal gold immunochromatographic method of AMOZ and AHD are as follows:The diameter of colloidal gold particles was 20 nm.The pH of colloidal gold solution was adjusted 9 and the optimal amount of antibody(2mg/mL)was 10 μL and 15μL.The dilution of gold labeled antibody was 1:5.The diluted solution of secondary antibody and coating antigen were PB,and dilution were 1:10.Millipore HF90s models of nitrocellulose membrane(NC membrane)don’t need to be blocked.The quantity of secondary antibody and coating antigen is 0.3 uL/cm.Detection time was 10 minutes.The limit of detection of colloidal gold immunochromatographic assay for the detection of NPAMOZ and NPAHD was 2μg/L and 30μg/L;The visual detection limit of the AMOZ and AHD in animal derived food were 0.3μg/kg and 4μg/kg.The optimal conditions of colloidal gold immunochromatographic method of AOZ and SEM are as follows:The diameter of colloidal gold particles was 20 nm.The pH of colloidal gold solution was adjusted 9 and the optimal amount of antibody(2mg/mL)was 10 μL,and 20μL.The dilution of gold labeled antibody was 1:8 and 1:6.The diluted solution of secondary antibody and coating antigen were PB,and dilution were 1:10.Millipore HF90s models of nitrocellulose membrane(NC membrane)don’t need to be blocked.The quantity of secondary antibody and coating antigen is 0.3 uL/cm.Detection time was 10 minutes.The limit of detection of colloidal gold immunochromatographic assay for NPAOZ and NPSEM was 5 μg/L and 10 μg/L;The visual detection limit of the AOZ and SEM in animal derived food were 0.5μg/kg and 0.8 μg/kg.