| In this paper, sulfamethazine to detect objects, exploring the use of electrochemical detection sulfamethazine of sulfamethazine in graphene electrochemical behavior modification have been studied, explored the electrochemical detection factors sulfamethazine as a new detection method provides a theoretical basis for sulfamethazine residues detected. Experimental results are as follows:1) Select the best working electrode to detect sulfamethazine from gold, platinum, glassy carbon （Au, Pt, GC） three kinds of electrodes, results showed sulfamethazine as the working electrode of glassy carbon electrode in electrical the most obvious chemical signals.2) Modified graphene material of the working electrode was measured by cyclic voltammetry （CV）, in contrast sulfamethazine different bare electrodes （Au,Pt,GC） and the graphene Electrochemical Behavior modification. The study found that the modified graphene material prepared by electrochemical sensors, the anode current response sulfamethazine improved significantly.3) The experimental conditions electrochemical detection of sulfamethazine screening and optimization, including support electrolytes, pH, scan rate and other parameters. Experimental results show that the best measurement of electrolyte sulfamethazine is pH=3.5 of 0.05mol/L acetate buffer solution, the oxidation peak current and scan rate in the 10-150mV/s range of linear correlation.4) Working electrode, a comparison of the two methods by modifying the dispenser and electrochemical deposition modification. The results showed that:the best dose of the modified electrode modified to drop coating 3μL, modified electrode electrochemical deposition made easy to fall off and drop better than modified electrode coating production.5) Square wave voltammetry（SWV）, chronoamperometry current method （i-t） of sulfamethazine solutions of different concentrations were determined. The results show that the square wave voltammetry to detect sulfamethazine, current signal and the concentration of 5.0×10-6～1.0×10-3mol/L good linear correlation coefficient R is 0.99512, the linear correlation equation is i （μA）=7.905+1.284LgC（μmol/L）, the detection limit is 3.0×10-6 mol/L; chronoamperometry current method （i-t） Determination of sulfamethazine, the current signal and the concentration of 7.0×10-7～5.9×10-5 mol/L good linear correlation coefficient R is 0.99921, the linear correlation equation is i（μA）=0.1245+0.01815xC（μmol/L）, the detection limit is 5.0×10-7 mol/L, chronoamperometry current detection limit is less than the square wave voltammetry.6) Use chronoamperometry current method of pig feed samples were measured current signal and the concentration of 3.0×10-7-2.3× 10-5 mol/L concentration range a good linear relationship, the detection limit is 5.3×10-9 mol/L the correlation coefficient R is 0.99843, linear equations i（μA）=0.29323+0.03086xC （μAmol/L）. |
