Aqueous Enzymatic Extraction And Refining Of Camellia Oleifera Seed Oil For Injection

Camellia oleifera seed oil is well-known as the“oriental olive oil”as the fatty acid composition is similar to olive oil which is a health protection senior oil and therefore they are widely used in medcicine and other fields.Using camellia seed as raw material in this study,camellia oleifera seed oil was extracted using ultrasonic-assisted aqueous enzymatic methods.Eleven vegetable oils were evaluated by principal component analysis and cluster analysis to select the optimal material for injection oil.Based on the results,processing parameters of camellia seed oil were optimized to reached requirement for injection oil,including alkaline deacidification,decoloration,and deodorization.The main points and contents are summarized as follows:Ultrasound-assisted aqueous enzymatic extraction of oil and protein from camellia was optimized by using the combination of Plackett Burman design and response surface methodology.Firstly,ultrasonic time,p H,response time were chosen by Plackett-Burman design as the main factors,then the extraction conditions were optimized by response surface analysis.The results showed that the optimal extraction conditions were: ultrasonic time 30 min,pH 9.0 and response time 3.5 h.Under the above conditions,the yields of oil was 89.704%,the yields of protein was 90.638%,with the predictive value error less than 0.5%.These results indicated the model build by combinedly using Plackett Burman design and response surface methodology was reliability.The composition of fatty acids and routine physical-chemical indexes of eleven oil(soybean oil,rapeseed oil,camellia oleifera seed oil,sunflower seed oil,olive oil,walnut oil,peanut oil,rice oil,inseed oil,maize embryo oil,maize sesame oil)were comprehensive analysed by principal component analysis.According to principal component load extracted five main components after regrouping,the contribution rate of the former 5 components are 35.447%,24.973%,11.038%,8.153%,6.336% which accounted for 85.946% of total variation.The characteristics of the principal component were analyzed,the regression equation related to principal component was seted up,and the regression equation is F= 0.412F1+ 0.291F2+ 0.128 F3+ 0.095F4+0.074F5.Those 11 kinds of oil’s composite scores were ranked,the camellia oleosa seed oil was higher,it is shows that camellia oleifera seed oil is suitable as raw materials oil for injection oil.Refine processing conditions of camellia seed oil were optimized to make the quality indexes of camellia oleifera seed oil reached requirements of oil for injection.(1)Deacidification :based on single factor tests,the optimization conditions for camellia oleifera seed oil deacidification by response surface method(RSM)was performed.The result showed that,the optimum parameters were the alkali refining temperature of 80℃,the alkali refining concentration of 12%,the alkali refining time of 60 minutes,and the ultra-alkali capacity of 0.16%.With the above processing,the acid value of oil-tea camellia seed oil was 0.0605 mg KOH/g and has excellent oxidative stability which was up to the standard of injection oil.(2)Decoloration:the optimization conditions for camellia oleifera seed oil adsorption decoloration by response surface method was performed.The optimum parameters were activated carbon mix with kieselguhr to the proportion of 1∶3,the decoloration temperature of 82℃,the decoloration reaction time of 35 min,and the mixed decolorizing agent loading is 3.5%.Under the best conditions,the bleaching rate was up to 92.461%,the color was stabled which difficult to be retumed and the camellia oil was up to the standard of medical camellia oil and cosmetic camellia oil.(3)Deodorization:useing steam deodorization for the oil,the optimum deodorization process conditions were vacuum 0.096 MPa,deodorizing temperature 230℃,time 2 h.All of the results suggested that not only the quality of refined camellia oleosa seed oil could reach the standard of injection oil accoding to Chinese Pharmacopoeia(2010 version),but also could provide important technical reference for the high level usage of other vegetable oils.