Development Of Technology For The Utilization Of Tilapia Steak

In this paper, we use the uselese parts of the processed freshwater tilapia-tilapia steak as the raw material. We get the fish on the steaks and fish bones separated;then studing the use of high-value fish and fish bone technology. We use lactic acid bacteria to deal with the fish bones, then collecting extract active calcium in the tilapia bones. On the other hand, we use protease hydrolysis to deal with the fish attached to tilapia bones, by doing so we get some hydrolysate; last we get aromatic amino acid removed by using activated carbon to refine high F value oligopeptide. Finally we tested the function of its activity by the way of caenorhabditis elegans model. The contents and results are as follows.1? We use tilapia bone as raw material; then use lactic acid bacteria to deal with the fish bones to collect extract active calcium in the tilapia bones.During the experiment, we try to find out what is the best experimental condition to collect the activity calcium. The results showed that: selecting the 6074 lactobacillus acidophilus, glucose 5g, peptone 0.5g,inoculation of 3m L/100 m L, tilapia bone meal 2g, water 100 m L, temperature 37?,fermentation time 48 h, the dissolution rate of calcium is up to 40%, content of amino nitrogen is up to 1.3 g / 100 m L.2? We use fish on the steaks as raw material, and then dealt it with enzymatic hydrolysis to get High Fischer ratio oligopeptides. By the way of single factor experiments and orthogonal test, we find out the optimal conditions for hydrolysis. Last we get aromatic amino acid removed by using activated carbon to refine the hydrolysis. It showed that:choosing alkalase 1200 U/g, ratio of fish to water 1:15, hydrolyzesed at 50 ?, p H 8.0, for4.5 h. In this hydrolysis the F value is 23.1, hydrolyzed protein recovery precent is 74.5%.The optimal absorption condition by activated carbon is ratio of activated carbon to hydrolyzate volume 1:15(g/m L), time 0.5h, p H 6.0, room temperature.In this hydrolyzate F value is 29.3, polypeptide recovery precent is 31.2%.3? The hydrolyzate is dealt with macroporous adsorption resin, and then graded elution with different concentrations of ethanol. So as to achieve the purpose of fractionation. Results showed that, using 60% ethanol elution to wash it at the speed of 1.5m L/min, we got the composition of the maximum value of F which is 19.3, peptide recovery precen is 23.2%, but it's F value fail to meet the requirements of high F value oligopeptide. At the same time we find that dealing hydrolyzate with the macroporous adsorption resin and the active carbon, which is that using the 60% ethanol elution to wash it at the speed of 1.5 m L/min, after activated carbon adsorping process.The F value is 31, it can significantly increased the F value, and meet the requirements of high F value oligopeptide.4? Two high F value oligopeptide component obtained by the hereinabove, F=22 and F=31(hereinafter referred to as Sample 1 and Sample 2). Using in vitro antioxidant activity evaluation method and explore the size of the F value relations with antioxidant activity.The results show: in vitro antioxidant activity evaluation of sample 1 and sample 2hydroxyl free radical clearance rate were 86.9%, 87.2%, there were no significant differences, preliminary specification sample 1 and sample 2 with antioxidant activity, but with the size of the F value and there is no direct relationship.5? We studied the influence of sample 1 and sample 2 on the longevity, spawning amounts, movement, and swallowing frequency of caenorhabditis elegans. The results show: sample 1 and sample 2 set of caenorhabditis elegans spawning amounts, swallowing frequency and movement significantly higher than that of blank group(P<0.05), and the positive control group had no significant difference( P > 0.05). there is no significant difference between sample 1 and sample 2. so the high F value oligopeptide can increase longevity, spawning amounts, movement, and swallowing frequency of caenorhabditis elegans of caenorhabditis elegans, but not directly related to the size of the F value.