Study On Preparation Polycaprolactone Grafted Modified Tannic Acid And Drug Loading Properties Of Blends Fiber

Tannic acid which is a kind of polyphenol compounds with complex structure has special physiological activity, and then it has been widely applied in the field of medicine. Electrospun biodegradable polyester fiber membranes attracted increasing attention because its high porosity, high surface-volume ratio and structure like extracellular matrix. However, the low biological activity of biodegradable fiber membrane limited it in medical application. In this study, we have synthesized polycaprolactone grafted tannin(TA-g-PCL) and studied on its related performances; Poly(lactic acid)/ tannic acid blending fiber membranes are made by electrospinning, and its related properties such as mechanics, wettability, cytotoxicity are investigated; Base on the above composite fibers, the methotrexate(MTX) loaded fibrous membranes are prepared for controlled drug release and Pluronic F-108(PF-108) is also incorporated into the medicated fiber to adjust drug release rate.1. Various polycaprolactone grafted tannic acids(TA-g-PCL) are synthesized via ring-opening polymerization of caprolactone(CL) monomer, of which the molecular weights are design as 8000, 15000, 30000, respectively. FTIR and 1H NMR prove successful synthesis of polycaprolactone grafted tannic acid. GPC shows narrow molecular weight distribution of the obtained TA-g-PCL. And the thermal properties and solubility of TA-g-PCL are closely related to the molecular weight of PCL.2. TA-g-PCL/ polylactic acid(PLLA) blending fiber membranes have prepared by electrospinning. The blending fiber membranes are characterized by using ESEM, FTIR, DSC, tensile, water contact angle and cytotoxicity. The effects of TA-g-PCL blending ratio on fiber morphology, thermal properties, mechanical properties and wettability are studied in detail. The results show that blending TA-g-PCL would change not only fibrous morphology, but also membranous wettability. The mechanics properties of fiber membranes change with blending ration of the TA-g-PCL adjusting, which shows the best performance at 15 wt% of TA-g-PCL. And the cytotoxicity experiment confirmed that the TA-g-PCL/ PLLA blend fiber membrane is non-toxic.3. Various fiber membranes loaded methotrexate(MTX) have been produced via emulsion- electrospinning. The various medicated fiber membranes are characterized by using ESEM, FTIR, DSC, tensile, water contact angle and in-vitro drug release. The effect of fibrous component and methotrexate content on the medicated fiber membrane including morphology, mechanical properties, wettability and in-vitro release behavior are disclosed. the results show that the fibrous components play a key role in the membranous performance, for example, blending TA-g-PCL and PF-108 into the medicated fiber membrane results in the release rate of MTX faster. Moreover, MTX content can also exert the influence on the membranous properties, such as the drug release behavior. The 5 wt% MTX/ 15 wt% TA-g-PCL/ PLLA blending PF-108 shows the best performance.