The Detection Method And Reagent Card Of Immunofluorescence For Myocardial Injury

Acute myocardial infarction with rapid onset,high of the mortality rate,strong before the onset latency and other characteristics,has become one of the most threatening diseases to human health in recent years. Cardiac troponin I(cTnI) and C-reactive protein(CRP) is an important series of markers of myocardial cells injury, for the rapid diagnosis of acute myocardial infarction has a very important significance.The research on human blood cardiac troponin I(cTnI) and C-reactive protein(CRP)were established two fluorescence immunoassay method based on quantum dots, developed two kinds of quantitative test strip.The main principle is to photoluminescence combined with the immune response, by double antibody sandwich method, a monoclonal antibody conjugated quantum dots made with a fluorescent marker, while the other targets of different monoclonal antigen antibody-coated onto a nitrocellulose membrane, both with the sample antigen to form a "sandwich" immune complex, marked a quantum dot emits red under UV excitation, relative luminescence units by detecting photons produced(RLU) whereby the quantitative detection of human serum cardiac troponin I and C-reactive protein.In this paper, the raw material of antigen-antibody screening and characterization of quantum dot mark, Immunochromatographic strip of construction and optimization methodology to assess other experiments.The results showed that the linear range of the test strip cTnI is 0.01 ng / ml ~ 0.5 μg / ml, total precision CV <5%; CRP test strip linear range of 0.1ng / mL ~ 1μg / ml, total precision CV <5%, the clinical sample test results show that with the imported reagent has good correlation, troponin I and C-reactive protein test strip performance indicators have already reached the level of similar imported reagents.Provide experimental foundation for the rapid diagnosis of AMI and test strip technology product development.