Fabrication Of PLLA/Coll/HA Electrospun Nanofiber Scaffold And The Effect On Proliferation Differentiation And Mineralization Of Osteoblast

As the bone defect has been attracted more and more attention. It is necessary to design multifunctional biomaterials that can induce and assemble bonelike apatite in bone tissue engineering. The major elements of natural bone are inorganic hydroxyapatite(HA) and collagen type I. It is significant to fabricate tissue engineering material by mimicking the structure, component and function of native bone tissue. This article mainly depended on the direction of simulation of natural bone tissue. We prepared different morphology of hydroxyapatites nanoparticles and characterized by scanning electron microscopy(SEM) and X-ray diffractometer(XRD). The biocompatibility and osteoconduction of HA were detected by MTT, alizarin red, and ALP. The composite materials of PLLA, PLLA/HA, PLLA/Coll, PLLA/Coll/HA were fabricated by electrospinning. The morphology, elements and surface hydrophilicity of the composite scaffolds were characterized by SEM, Electronic Differential Systemwater(EDS) and water contact angle. The effect of the nanofiber scaffolds on proliferation, differentiation and mineralization of MC3T3-E1 cells was investigated. The results show that we prepared nanoparticles was nanorods and sphere. In vitro studies clearly showed that HA of sphere exhibited superior osteoinductivity compared with HA of nanorod. So we used HA of sphere blending with PLLA and Coll. The size of PLLA, PLLA/HA, PLLA/Coll, PLLA/Coll/HA were uniform and in the range of 300-400 nm. The result of water contact angle indicated that collagen improved the hydrophility. In vitro studies clearly show that the scaffold of PLLA/Coll/HA exhibited superior osteoinductivity compared with other scaffolds.