Synthesis And Characterization Of Gelatin-based Drug Loaded Elastomer Nanocomposite

With the growing population of the world, the social resources get more and more scare, especially these synthetic materials from non-renewable petrochemicals.Therefore, biological materials especially natural biodegradable and absorbable materials get more attention in recent years. In addition, nanotechnology has also matured and conjugated biological materials with nanotechnology have been for exploration in many applications.Gelatin, derived from animal collagen, non-toxic, biodegradable, biocompatible, non-immunogenic, has been used in the field of medicine, food and cosmetics for a long time. Based on the aim of biomedical application, furthermore, aiming at developing wound dressing membrane, gelatin based drug loaded elastomer nanocomposites were fabricated. The structural and biomedical properties of the membranes were characterized. The development of gelatin based material can solve the waste problem and realize the vision of green chemistry.Based on the above factors into account, we chose gelatin as matrix material, glycerol as plasticizer, two different antimicrobial drugs including polymyxin B sulfate and ciprofloxacin which have synergistic effect, halloysite nanotubes as a reinforcing material as well as the drug delivery carrier, genipin as bio-crosslinking agent and then we prepared gelatin-based drug loaded bio-elastomer nanocomposite by hot melt HUCK blending.A variable of gelatin-based drug loaded bio-elastomer nanocomposite was fabricated by adding different content of glycerol to explore the influences of glycerol to the properties and structure ofmembranes. Based on the results of physical and chemical properties, biodegradable and water resistance properties,gelatin matrix and the nanoparticles with different morphology had good interaction. Nanoparticles dispersed well in the gelatin matrix.The nanocomposites with 150 phr glycerol possed better property than nanocomposite with other content of glycerol.Based on the last part, a series of gelatin-based bio-elastomer nanocomposite with 150 phr glycerol,10 phr drug of ciprofloxacin and different concentrations of drug loaded halloysite (0,5,10,15, and 20 wt% polymer) were prepared. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that nanoparticles are nearly not aggregated. FT-IR and X-ray diffraction results show that the addition of nanoparticles did not change the structure of the material itself. We can see that with the increase content of halloysite nanotubes, the tensile strength of the nanocomposites improved to nearly 2MPa and reduces the rate of thermal degradation. All the nanocomposites presented non-toxic but not good biocompatibility and cell adhesion and proliferation in vitro bio-experiment. In vitro drug release results showed that two drugs released and could produce antimicrobial effects to prevent the colonization of P.aeruginosa and S.aureus.To investigate the effect of HNT to the drug release behavior, the nanocomposite membrane added directly with CPX, HNT, polymyxin B sulfate into gelatin/glycerol was fabricated. Compared with the membrane with polymyxin B sulfate loaded halloysite nanotubes, these membranes with polymyxin B sulfate and halloysite nanotubes apart released drug more continuously. And the bacteriostasis test can verify the same result which can prove that halloysite nanotubes have the influence of extending release of drug.Gelatin-based bio-elastomer nanocomposite used in biomedical field, it can not only keep te characteristics of gelatin but also can add more excellent property with the nanoparticles. Meanwhile, these material can achieve the win-win of both performance an environment.