The Effects Of Lactobacillus Plantarum NCU116 On Intestinal Health

Probiotics are live microorganisms that can provide benefits to human health when administered in adequate amounts. Studies have demonstrated that probiotics have several kinds of functions, such as regulating intestinal flora, improving intestinal mucosal barrier, alleviating metabolic syndrome and immunomodulatory. Lactobacillus plantarum NCU116(NCU116) was recently isolated from pickled vegetables. Previously reports have showed that this bacterium is characterized with good performance in vitro and in vivo. However, the character of the bacterium in the intestinal tract in vivo is unclear. The present study was to investigate the effects of NCU116 on mice intestinal immunity, intestinal mucosa damage, intestinal flora and metabolic disorders in cyclophosphamide(CTX) treated mice, and elucidate the function mechanism underlying NCU116 regulating effects. The main conclusions were summarized as follows:(1) All mice were randomly divided into six groups as follows: orally administrated three doses NCU116 groups(NCU-H, 1010 CFU/mL; NCU-M, 109 CFU/m L; NCU-L, 108 CFU/m L, respectively), normal control group(NIM), model control group(IM) and administrated with 109 CFU/mL Bifidobacterium BB12 as positive control group(BB12) for 20 days. Compared with the IM group, intestinal immune, intestinal flora and intestinal metabolism were improved in the groups that received NCU116. In addition, the bacterium reduced the intestinal mucosal injury and increased the mucins expression.(2) This part of study examined the effects of NCU116 on intestine mucosal immunity in CTX-induced immunosuppressed mouse model. Our results showed that supplemented with NCU116 significantly increased the immune organ index, the number of immune cells and the levels of cytokines secretion and mRNA expression on the immunosuppressed mice. In addition, the NCU116 have stimulative effects on some toll-like receptors and increased its expression levels. Further study results showed that the NCU116 has regulating effects on the balance of Th1/Th2 and Th17/Treg cells differentiate, it could be an important signaling pathway of NCU116 to regulate the intestinal immunity to form a specific intestinal immune environment. In conclusion, the present study has demonstrated that NCU116 could improve the immune on the immunosuppressed mice.(3) This part of study examined the effects of NCU116 on intestinal mucosal injury in CTX-treated mice. Our results showed that supplemented with NCU116 significantly improved the intestinal mucosal morphology, and promoted the growth of intestinal epithelial cells for increasing the villus height and crypt depth. In addition, our results also showed that the NCU116 increased the intestinal mucosa structure integrity and effectively enhanced the defensive ability of the intestinal epithelial by promoting goblet cell proliferation and secretion of mucin proteins. In conclusion, the present study has demonstrated that NCU116 could attenuate and repair the intestinal mucosa damage. Combined with related studies, the NCU116 on the improvement of the intestinal environment may be significantly related to intestinal mucosal repair.(4) This part of study examined the effects of NCU116 on intestine flora and intestine metabolism in CTX-treated mice. Our results showed that supplemented with NCU116 improved the intestine microbial community by increasing the counts of Lactobacillus and Bacteroides, and decreasing the counts of Enterobacterium, Pseudomonas and Clostridium. In addition, the NCU116 has effects on attenuates intestinal metabolic disorders by increasing the level of short-chain fatty acid and reducing the production of ammonia in the colon feces, and the pH value significantly deceased in all the NCU116 treatment groups. In conclusion, the present study has demonstrated that NCU116 could improve the intestine microbial community and intestinal metabolism. Combined with related studies, our findings elucidate the molecular basis underlying the effect of NCU116 on the intestinal metabolism through its interactions with the intestine microbial community.(5) Evidence from our study clearly indicated that the administered of NCU116 could be beneficial to intestinal immune, intestine mucosa, intestinal flora and intestinal metabolism. The intake of NCU116 also can modulate the intestinal immune and intestinal metabolism through its interactions with the intestine microbial community. Sequentially, intestinal metabolites had effects on intestine mucosa and intestinal immune.