Design And Application Of PET-based Reactive Fluorescent Sensors

Since the 21th century, along with the development of technology and bioscience, people put forward higher requirements for detection technology. Fluorescent probe method, as a kind of biological detection technology, is becoming an effective tool for localization, molecular recognition and dynamic monitoring of biomolecules, due to its high selectivity, sensitivity, operational simplicity and real-time monitoring capabilities. The design and application of fluorescent probes have recently become one of the research hotspot.Low molecular weight biothiols, such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), are components of many petides that play key roles in the pathogenesis of serious diseases. Therefore, the detection of trace biothiols will be of importance to medical sciences. We have utilized naphthalimide to synthesis a novel biothiol fluorescent probe (TC) which has outstanding two-photon performance and low cytotoxicity. It can avoid the background fluorescence interference of the biological samples effectively during the use of two-photon microscopy. Importantly, fluorescent probe TC that response to biothiols specifically in the lysosome is of interest to elucidate organelle functionality.a-Ketoglutarate is one of the most important nitrogen transporter in the metabolic pathways. The somatic mutation of isocitrate dehydrogenase in the cytoplasm can make a-ketoglutarate into 2-hydroxy glutaric acid, moreover, the concentration of a-ketoglutarate in the tumor of acute myeloid leukemia patients will decrease. Therefore, the accurate detection of a-ketoglutarate will be a significant role in promoting the early diagnosis of diseases. In order to realize a "turn-on" fluorescence response towards a-ketoglutarate, a kind of fluorescent probe sz with naphthalimade-based conjugating hydrazine as a identify group. Probe sz has a good selectivity towards a-ketoglutarate without interence from other reactive amino acids. Moreover, the sensitivity of probe sz towards a-ketoglutarate is very high with a detection limit of 0.163 ?M.