Study On The Preparation Of Antioxidative Peptides From Corn Gluten Meal And Microcapsulation

ObjectiveCorn gluten meal is a main by-product by produced corn starch system,which with poor water solubility,special smell of corn fermentation,influencing its application in food industry.At present,corn gluten meal are generally processed into fodder or as the liquid wasted to emission.For reduce the waste of corn gluten meal,and protect environment.In this paper,corn gluten meal as research object to refining and product development,as well as to promoting the application of corn gluten meal.MethodsCorn gluten meal gliadin was hydrolyzed by Alcalase protease to prepare antioxidant peptides.On the basis of the single factor experiment,enzyme concentration(E/S),pH and hydrolysis time were identified as main affecting parameters,the scavenging activity of DPPH free radicals as response value for evaluation of response surface analysis.To obtain the optimal hydrolysis conditions.Studying the decolouring of physisorption antioxidative peptides solution,Single factor experiments were done to explore the effects of decoloration rate and the nitrogen recovery of active carbon,pH,temperature and time.Using the orthogonal experiment to obtain the optimum process.The best macroporous resin was used to desalt the permeate obtained byantioxidative peptides.The static adsorption of the best macroporous resin and the static desorption of antioxidant peptides with different time and desorption solvents were performed under different pH and time conditions.Study on dynamic adsorption of macroporous resin with different concentration and flow rate.After dynamic adsorption,the macroporous resin was eluted by ethanol with different concentration,and then collected the eluent,to determinated of desalting ratio after the vacuum freezed drying.The piercing method preparation of corn gluten meal antioxidant peptide microcapsulation.Single factor experiments were done to explore the effects of sodium alginate concentration,mass ratio of core to wall material,temperature and calcium chloride concentration,and the microencapsulation efficiency as response value.On the basement of Single factor experiments,and using response surface analysis Box-Behnken design to obtain the optimal the optimum process.Finally,researched and evaluated the antioxidant activity and properties of storage.Results1 ﹑ The optimal hydrolysis conditions were determined as enzyme concentration(E/S)of 8.00%,hydrolysis pH of 9.30,hydrolysis temperature of52℃ and hydrolysis time of 4h.Under these conditions,the scavenging activity of DPPH free radicals was up to(83.06±0.46)%.2 ﹑ The high level of discolouration can be obtained under the following condition: pH5.0,active carbon 3.0%,temperature 70℃,time 60 min,under this condition,the decoloration rate was(35.17±0.01)% and the nitrogen recovery was(79.82±0.09)%.3﹑Peptide was desalted by DA201-C macroporous resin,and the best parameter were pH5.0,10mg/m L and 1.0m L/min.After adsorption,the macroporous resin was eluted by concentration of 70% showed the best antioxidant activity in the DPPH scavenging activity and the reducing capacity.Under this condition,the desalt ratio was(95.13±0.07)%,the recovery of antioxidant peptides was(84.49±0.03)%.4 ﹑ The results showed that the optimal experimental conditions were sodium alginate concentration 2.0%,mass ratio of core to wall material 1:1.5,temperature 50℃ and calcium chloride concentration 2.0%.Experiments under these optimal conditions led to a microencapsulation efficiency of(78.51±0.17)%,5﹑The antioxidant activity and properties of storage of microcapsulation:Study on microcapsulation and raw material’s antioxidant activity in the DPPH scavenging activity and the reducing capacity with the same concentration.Although the microcapsulation’s antioxidant activity was slightly lower,it also had antioxidant activity.The embedded peptide had better antioxidant activity than which was not embedded at room temperature for 30 days.The temperature shows that microcapsulation was a good method for improving the stability of antioxidant peptide.ConclusionsIn this thesis,corn gluten meal was hydrolyzed by Alcalase protease to prepare antioxidant peptides,on the basis of single factor experiment,using the response surface to optimize the hydrolysis conditions,the optimal process parameters is enzyme concentration(E/S)of 8.00%,hydrolysis pH of 9.30 and hydrolysis temperature of 52℃.Under these conditions,the scavenging activity of DPPH free radicals was up to(83.06±0.46)%.In order to obtain the high purity of antioxidant peptides,the antioxidant peptides was decolorized as well as desalted,with the activated carbon,the decoloration rate was(35.17±0.01)%and the nitrogen recovery was(79.82±0.09)%.The antioxidant peptide was desalted by DA201-C macroporous resin,the desalt ratio was(95.13±0.07)%,the recovery of antioxidant peptides was(84.49±0.03)%,and obtained the high activity antioxidant peptides.The piercing method preparation of corn glutenmeal antioxidant peptide microcapsulation,on the basement of Single factor experiments,and using response surface analysis obtain the optimal the optimum process is sodium alginate concentration 2.0%,mass ratio of core to wall material 1:1.5 and calcium chloride concentration 2.0%.Experiments under these optimal conditions led to a microencapsulation efficiency of(78.51±0.17)%.The embedding antioxidant peptides microcapsules products also have antioxidant activity in the DPPH scavenging activity and the reducing capacity,and the experiment showed that the embedded antioxidant peptides had better antioxidant activity than which was not embedded at room temperature for 30 days.