| Objective:Explore the best ratio of Cassia lipid-lowering compound beverage with coffee to make a nice flavor, and to verify the effectiveness of lipid-lowering compound beverage.Methods:1. Selection cassia with high quality, and identify medicinal herbs; use oven baked cassia,make sure the fumed cassia’ appearance, color, aroma were recorded, to find a suitable fumed time, temperature, thickness; use powder machine and hole sieve, hit the baked Cassia to powder and filtration several rounds; using boiling to brew pure cassia powder, exploring the amount of the minimum and maximum tolerated dose flavored by color, taste, etc.; using a three-factor orthogonal experiment,per 100 ml water, according to the cassia powder(A1: 0.3g, A2: 0.35 g, A3: 0.4g),coffee(B1: 0.8g, B2: 0.85 g, B3:. 0 9g), coffee mate(C1: 3g, C2 : 4g, C3: 5g), Each of which has unequal ratios; develop flavor assessment programs for different proportions of composite beverage flavor assessment, Looking for the best ratio.2. The establishment of mouse model of hyperlipidemia, The 100 KM mice were randomly assigned divided into control group X, fat-positive group Y, hyperlipidemia prevention group Z, X, Y, Z number three groups of mice were 30, 60, 10.X control group fed normal diet, Y positive group fed high-fat diet, Z prevention group fed high-fat diet at the same time, each mouse orally with 250 mg / kg solution of cassia powder per day.After four weeks, 10 mice were stochastic elected out from the group,arterial blood, checking concentration of five indicators in the serum,like HDL, LDL,TC, TG, s LOX-1; take liver, after HE staining of liver, placed in the optical microscope.3. After the model is successful, the remaining 20 mice of X group were randomly and average divided into control group A and maximum dose toxicity groups G, A group and G group continued feeding normal diet.Y group 50 mice werestochastic and average separated into fat positive group B, positive drugs control group C, D is 1 times the same amount of powder cassia group, E is cassia powder 5times the same amount of group, F is 10 times the same amount of powder cassia group.B, C, D, E, F five groups carry on feeding high-fat diet. Group C per mouse daily orally with 270mg/kg Zhibituo piece solution, D group per mouse daily orally with 50mg/kg cassia powder solution, E group per mouse daily orally with 250mg/kg cassia powder solution, F group per mouse daily orally with 500mg/kg cassia powder solution.Four weeks later, within 48 h, group G each mouse will orally with5000mg/kg powder solution cassia three times(100 times of recommended dosage of Cassia powder). Thereafter, the 70 mice blood from aortic, checking concentration of five indicators in the serum,like HDL, LDL, TC, TG, s LOX-1.Take renal andliver,liver and kidney tissue was observed for morphological tissue changes under HE staining.Detect changes in blood cells of A、B、D、E、F.Results:1. Cassia Pharmacognosy identified as legumes, caesalpinoideae family, Cassia,Cassia, select cassia with full particles, bright color. The Cassia’ optimum temperature baked with oven was 150 ℃, up and down the hot steam, the recommend appropriate duration was 6min, thickness was 2 ~ 3mm. Appropriate order for collecting powder cassia was play to powder 3~4min at first, through 200 mesh screen following.Calculated by 100 ml water, adding 0.05 g cassia powder with a touch of flavor, when reaching 0.6g, color too thick, bitter heavier. Using a three-factor orthogonal experiment and flavor assessment,we can found the best ratio is A2B3C1,that is cassia powder 0.35 g, coffee powder 0.9g, coffee companion 5g.2. Compared with normal group X, Y model group serum TC、TG、s LOX-1increased significantly(p<0.01), HDL decrease significantly(p<0.01), LDL also increased visible(p<0.05).Compared with normal mice,the high cholesterol mice’ liver hypertrophy under general observation, HE staining below light microscope, uneven distribution of cells within slices steatosis occupy the lobule 1/2 to 2/3. There are some cells to increase orswelling,vacuoles in the intracytoplasmic was filled with lipid droplets, it indicates successful model.In the light of the concentration of s LOX-1, There was significant between group X and group Y(p<0.01), there was significant between group Y and group Z(p<0.01).In the light of the chroma of LDL, There was significant between group Y and group X(p<0.05), There was no significant between group Y and group Z(p>0.05). In the light of the chroma of HDL, there was significant between group Y and group X(p<0.01), there was significant between group Y and group Z(p<0.01). In the light of to the chroma of TC, there was significant between group Y and group X(p<0.01), there was significant between group Y and group Z(p<0.01).In the light of the chroma of TG, there was significant between group Y and group X(p<0.01),there was significant between group Y and group Z(p<0.01).3. Compared with the normal group A, model group B, HDL hadevidently decreased(p<0.01), TC、TG、s LOX-1、LDL were increased significantly(p<0.01).Drug-energetic group C,there were not statistically significant with TC、HDL(p>0.05), TG, s LOX-1, LDLwere statistically significant(p<0.01).Group D,there was no significant with TC、HDL(p> 0.05), TG、s LOX-1、LDL changes were statistically significant(TG、s LOX-1: p<0.01, LDL: p<0.05).Group E and group F in s LOX-1 was statistically significant(p<0.01), there were not statistically significant with TC、TG、HDL、LDL(p> 0.05).Compared with the model group B, group C, group D, group E and group F in TC, TG, s LOX-1, LDL, HDL changes were statistically significance(p<0.01).Compared with the normal group A, group D、group E、group F and group G of hemoglobin levels and red blood cell levels changes were not significant(p>0.05).White blood cell count were higher, with group A group F, group G, the difference was significant(G:p<0.05, F:p<0.01).Conclusion:1. baked properly Cassia powder has similar coffee aroma, with coffee powder and coffee mate, the flavor is very appropriate. It will be welcomed by the vast ofconsumers, there is a good market potential.2. Through the flavor assessment, we have succeed outcrop suitable ratio of three factors in order to serve the further development.3. Cassia has a good preventive effect and reduce high blood cholesterol, with high security, can be made to be medicine and edible.4.s LOX-1 relative lipid four indicators, with greater sensitivity, can be used as a reference index diagnosis of high cholesterol. |
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