Preparation And Evaluation Of The Molecularly Imprinted Polymers Under Molecular Crowding Conditions

Objective Molecular imprinting is a new technique for synthesizing material of molecular recongnition with the ability of pre-selectivity.Molecularly imprinted polymers(MIPs)are prepared by copolymerizing functional and cross-linking monomers in the presence of a template.After the removal of the template,the synthesized MIPs possess micro-cavities with a three-dimensional structure complementary in both shape and chemical functionality to that of the templates,and are able to rebind the templates.Up to date,they have been successfully used in many fields.Molecular crowding,a new cencept of molecular imprinting,was applied to improve selectivity and capicity of MIPs.Method In this work,with methacrylic acid(MAA)as functional monomer,ethylene glycol dimethacrylate(EDMA)as cross linker,polystyrene as molecular crowding agent,an imprinted polymer was synthesized by precipitation with S-naproxen as template.At the same time,in this work,with methacrylic acid(MAA)as functional monomer,ethylene glycol dimethacrylate(EDMA)as cross linker,polystyrene as molecular crowding agent,an imprinted monolith was synthesized by an in-situ polymerization with enrofloxacin as template.Result The results of drug release indicated that the novel MIPs prepared with molecular crowding have better sustained release effect than that prepared with non-crowding agent.The results of fitting of release curve suggested that adding molecular crowding agent in porogen may lead to reversed release mechanisms.The results of chromatographic analysis indicated that the adding of PS increased the binding sites and the selectivity of resulting MIPs.Conclusion The present study showed that molecular crowding is an effective approach to enhance the effect of molecular imprinting.