Preparation And Osteoinductive Efficacy Study Of Organic Strontium Coating

To deal with long recover time for bone defect healing and interface problem, a superior osteogenic scaffold material must be applied. Acellular bone matrix has a natural support structure and bone induction property, which can improve the bone transplantation and formation. Titanium and titanium alloy has good mechanical properties and biocompatibility, which can be widely used for bone grafting material. In order to make full use of the natural scaffold structure and its osteoinductivity of acellular bone matrix, a coating with biological activity and stability must be prepared on the surface of acellular bone matrix. To promote the bioinert and the bone repair ability of titanium and titanium alloy, the surface of titanium and titanium alloy must be modified. In this work, we adopt a surface chemical method to prepare an ACBM-Sr coating on the surface of acellular bone and titanium alloy.The morphology and composition of the ACBM-Sr coating on the surface of acellular bone matrix were characyerized by SEM, XPS, FTIR and HNMR. The curve of Sr release was monitored by ICP-OES. Cell biology experiments of ACBM-CMCA, ACBM-0.1%Sr and ACBM-0.5%Sr results indicate that live cell ratio of the three kinds of coatings are significantly higher than that of dead cell ratio. The results also show that the coated acellular bone can support the cell survival. With prolong the cell culture and increase the content of Sr in the coating, cell number gradually increased and the size of cell gradually growed up. Cell proliferation of the ACBM-Sr coating on the surface of acellular bone was detected by using Alama blue. The results indicated that Sr can promote the proliferation of cells. Moreover, with increased the content of Sr, the cell proliferation rate was increased.The morphology and composition of the ACBM-Sr coating on the surface of titanium and titanium alloy were characyerized by SEM, XPS, FTIR and HNMR. The curve of Sr release was monitored by ICP-OES. The ACBM-Sr coating evenly distributed on the surface of titanium and titanium alloy. The morphology of MC3T3-E1 on Ti and Ti-Sr indicate that their surface support the adhesion of MC3T3-E1 cells, and the cells in good condition.