Preparation And Application Of Weak Acid Cation Stationary Phase For Ion Chromatography

Ion chromatography (IC) is a well-established analytical technique and has been one of the most effective tools for the determination of ions species for the past 30 years or more. As the same as other chromatographyic techniques, columns are the hearts of IC. Therefore, studying on preparation of novel ion chromatographic stationary phases has attracted the interest of scientists. With its rapid development of application domain, IC faces the challenge of samples and target ions being more and more complicated. Hence, the development of new ion-exchangers is more and more important. In this paper, we developed a polymeric substrates weak cation chromatographic stationary phase for the separation of organic amines using non-suppressed conductivity detector. The main research content is composed of the following three chapters.In Chapter 1, the development, classification and main application of IC had been introduced. Research progress of anion and cation stationary phase were discussed. Then the preparation of all kinds of stationary phases was reviewed.In Chapter 2, the functional chloracetylated polystyrene-divinylbenzene (PS-DVB) substrates were prepared by Friedel-Crafts acetylation on PS-DVB. The modified PS-DVB was based on the reaction of Iminodiacetic acid (IDA) with chloracetylated polystyrene. The scanning electron microscope (SEM), elemental analysis, breakthrough curves and the separation of organic amines were utilized to study the surface morphology, elemental contents, exchange capacity and chromatographic separation performance. Finally, the homemade column was used for the determination of isopropylamine in the glyphosate isopropylamine.In Chapter 3, copolymerization of methyl methacrylate with the remaining double bond on the surface of PS-DVB was to introduce a large number of epoxy groups on the surface of the microspheres. The modified PS-DVB was based on the ring-opening reaction of Iminodiacetic acid (IDA) with PS-DVB. The scanning electron microscope (SEM), elemental analysis, infrared spectrum, breakthrough curves and the separation of organic amines and cations were utilized to study the surface morphology, elemental contents, exchange capacity and chromatographic separation performance.