The Experimental Study On The Growth And Reproduction Characters Of SRB Domesticated With New Carbon Sources And Treatment Of Acid Mine Drainage

Acid mine drainage (AMD) contains lots metal ions and sulfate, the problem of wide pollution, low effective control and great difficuly are existed. This paper studiedsulfate-reduction bacteria (SRB) domiticated with new carbon sources, identification, the growth rule of SRB and reaction kinetics of removing sulfate, the reaction factors of removing sulfate and the effect of removing heavy metal ions (Fe2+, Mn2+, Cu2+, Zn2+). On this basis, futher research on absorbing by bentonite compositepartciles combined with microorganism reduction to deal with AMD through dynamic test, The aim is to explore a complementary and high efficient, economic and environmental protection treatment technology.Based on previous reasearch, to determine the best scale of 80:7:3, anaerobic fermentation 15 days fermented liquid can be used as excellent new organic carbon source of SRB. The strain is identified as SRB genus by staining the SRB which domesticated by new organic carbon sources. Experiment of the growth and reproduction law about SRB studies show that:the growth retardation of SRB is 0-1 day, growth logarithmic is 1-6 d day, period of stability is 6-9 day, demise is 9 day late; and microorganism into the logarithmic growth phase, and when F/M value>7, logarithmic phase,3<F/M value<7, stable period, F/M value<3 decline period, and to determine the specific growth rate of SRB, its u law change with time, among them the maximum specific growth rate:umax=1.2/d, established the sulfate reaction kinetics of SRB is v= 0.0386S/(760+S).Based on previous reasearch to determine the optimum reaction conditions for removing sulfate by SRB domesticated with new organic carbon sources. The optimum reaction conditions could be reached at inoculation amount of SRB was 8% under anaerobic condition,35 ? growth temperature,50 r/min rotational speed,1.5-2.0 C/S ratio,6-7 pH value, initial concentration of sulfate lower than 3000 mg/L, range of concentration of Fe2+ 100-300 mg/L and the concentration of Mn2+(?35 mg/L) and the concentration of Cu2+(?15 mg/L) and the concentration of Zn2+(?45 mg/L). Under that conditions the efficiency of removing sulfate could reach up to 90%. It has good removal effect on metal ions (Fe2+, Mn2+, Cu2+, Zn2+), the removal rate is over 95%. Reasarch on metal ions (Fe2+, Mn2+, Cu2+, Zn2+) toxic effects on SRB. The growth of SRB was inhibited at the concentration of Fe2+(?600 mg/L) and Mn2+(>140 mg/L) and Cu2+(>15 mg/L) and Zn2+(>45 mg/L). When the concentrations of Fe2+, Mn2+, Cu2+, Zn2+ respectively is 100,35,15,30 mg/L coexistence, coexisting competitive adsorption.In order to give full play to bentonite composite particles released alkalinity neutralize acid adsorption of heavy metal ions excellent properties and with SRB combination to realize synchonous sulfur, heavy metal ions removal, acidity reduction purpose. Contrast dynamic experimental study on absorbing by bentonite composite particles combined with microorganism reduction to deal with AMD. The results show that:Adsorption of bentonite composite particles combined with microorganism reduction to the removal effect of AMD better than single microorganism. Bentonite composite particles and biological carrier using layered way of packing removal efficiency better than no layered.5:5 of layered dynamic columns to the removal effect of AMD is best in five dynamic columns, reveals that after bentonite adsorption of heavy metal ions is conducive to microorganism reduction of sulfate produced S2" can be further fixed heavy metal ions.and make bentonite composite particles get regeneration, which can realize the collaboraative purification effect, it is an innovative and efficient efficient water treatment method, it is worth to application and spreading.For the residual COD, NH3-N, P treatment research, adopt the filled with activiated charcoal and zeolite welland treatment effect is good.