| With increasing depth of coal mining in China, traditional gas drainage methods for gas treatment could not completely meet the needs of production safety, and the low utilization of gas caused not only environmental pollution but also energy waste, so developed new gas control technology to guarantee the security and protection the energy environment was urgently needed. This article was intended to undertake a study on utilization of microbial technology for gas treatment in mine, and its feasibility on underground gas-control was demonstrated through a large number of experiments.Based on the understanding of physiological features, survival distribution and principles of degradation gas about methanotrophs, the author collected three kinds of soil samples from rice filed, Longyuan Lake and Fishing Ditch, then successfully separated methanotrophs from them. Subsequently their physiological characteristics were studied and the stain separated from rice field was found most actively, at the same time the most suitable conditions for its nurturing were studied. After that, gas degradation capacity of this strain was confirmed through the monitoring of gases in training bottle, and then its degradation effectiveness, degradation range of gas concentration and oxygen situation required were explored. Experiment results indicated that methanotrophs could remove environmental methane well, especially for low-concentration methane which could be removed rapidly.Thereafter, combined with high-pressure isotherms adsorption experiment, gas degradation capacity of methanotrophs was studied under simulated coal seam with high gas pressure and low-oxygen environments. Results showed that the strain could remove methane in these environments and promoted gas desorption at the same time.The average oxidation rates could up to 186mLCH4?h-1?L-1culture of 3d in relatively proper conditions. Finally, the specific approaches of using methanotrophs to control gas were discussed after feasibility demonstration. |
