The Preparation And Properties Of Targeted HCPT Lipid Nanoparticles

The application of free drug in clinic was limited by many defects such as bad absorption,poor targeting,fast metabolism,short action time and side effects,et al.Many efforts have been made to devise a high-performance drug delivery system with the aim of against the multidrug resistance(MDR)as well as increasing the targeting ability.In the present study,HCPT loaded solid lipid nanoparticles(SLN/HCPT)were prepared by using glycerol esters,as the lipid core,and tamarind seed polysaccharide,as the shell material.The SLN/HCPT was characterized for size,entrapment efficiency and drug release manner.The cytotoxicity of SLN/HCPT was assessed in vitro using HepG2/HCPT cells and in vivo ultilizing human tumor xenograft nude mouse model.SLN/HCPT had the ability to target HepG2/HCPT cells and accumulated higher drug content in HepG2/HCPT cells.The cytotoxicity of SLN/HCPT was concentration-dependent.SLN/HCPT showed enhanced cytotoxicity to HepG2/HCPT cells than HCPT injection.Based on the above research,nanostructured lipid carriers(NLC)coated with xyloglucan was prepared by soya oil and xyloglucan consisting of side chains with galactose residues,a terminal moiety that can be used to target HCPT to hepatoma.The therapeutic potential of NLC/HCPT was investigated on HepG2/HCPT cells and on human tumor xenograft nude mouse model.NLC/HCPT not only indicated superior cytotoxicity against the HepG2/HCPT cells but also in vivo,generated a higher therapeutic effect.Systemic toxicity study demonstrated that the carrier reduced systemic toxicity.NLC/HCPT proved a great potential to serve as DDS to against MDR of HepG2/HCPT cells.These results suggested that SLN/HCPT and NLC/HCPT represent a promising carrier for drug delivery to the hepatoma and reverse the drug resistant of HepG2 cells and XG could be exploited as a potential targeting device for liver tissue.