Effects Of Arabidopsis Thaliana TRANSPORTIN1 On Stem Cell Homeostasis And Screening For Interaction Proteins

The TRANSPORTIN 1(AtTRN1)gene of Arabidopsis is thought to encode a nuclear transporter protein,and its function may be similar to its homologous protein IMP beta 1(yeast)and hnTRN1/2(human cells),which identify the specific substrate proteins through a certain sequence of amino acid sequences and transport them from the cytoplasm to the nucleus.Participate in all kinds of life activities.However,the function of the protein is more than that.Recent studies have found that AtTRN1 affects the activity of the microRNA by promoting the combination of ARGONAUTE 1(AGO1),but does not involve the nuclear-cytoplasm distribution between the interaction protein AGO1 and microRNA,indicating the diversity of its functions.Although their main function is nuclear transfer(for example,about 60% of hnTRN1 interactived protein was used as transport substrate),it also participates in many other biological processes,such as regulating the assembly of the spindle and the nuclear membrane during mitosis,affecting the formation of the cilia and stress anti stress particles.The phenotypic analysis of the AtTRN1 deletion mutant trn1-3 showed that the trn1-3 apical meristem(shoot apical meristem,SAM)and the apical meristem(root apical meristem,RAM)developed abnormally,SAM is smaller,and the root is short,indicating that it is very important to maintain normal development of the plant.In order to understand how At TRN1 affects apical meristem development,we have found that some of the identification genes of apical meristem,such as CLV3,WUS,STM,and so on,have changed to a certain extent by gene chip and in situ hybridization.But how does AtTRN1 affect these signaling pathways,so we hope to screen more proteins that interact with At TRN1 through the yeast screening library,understand more biological functions of AtTRN1,and provide new directions for subsequent experiments.This study will help us further understand At TRN1 and how to maintain the top meristem stem cell homeostasis.