The Chromosome Conformation Analysis Of Mouse Cells By Fluorescence Imaging And Skeleton Extraction

Chromosome as the carrier of important genetic material in life,its spatial configuration(namely chromosome structure,number and position of centromere)of which has a close link with the fundamental biological processes in cell such as gene expression and epigenetic inheritance.Chromosome abnormality or mutation may lead to abortion and many diseases in pregnant women(such as congenital deafness,mental retardation,cat's call syndrome,male testicular abnormality).Therefore,the analysis and study of chromosome conformation is one of the most important tasks to improve the quality of life of life.In order to realize the accurate and quantitative analysis of chromosome conformation,this paper presents a framework separation method for optical imaging equipment to collect too much jumbled information.Based on the cytoskeleton theory,the skeleton extraction of two-dimensional chromosomes is carried out.The topological structure of chromosomes was extracted by binary transformation and distance transformation to reduce redundant information and the quantitative characterization parameters of chromosome skeleton were analyzed.The results showed that the chromosome skeleton morphology of bone marrow cells of ICR female mice included X,V and U types,which were classified according to the morphology of silk particles.The results showed that there were 40 chromosomes in the bone marrow cells of ICR female mice,ranging from 2.69 to 5.74 micron in length,of which 35% were centromere particles,20% were near centromere particles,5% were near telomere particles and 40% were telomere particles.Then,the skeleton of three-dimensional chromosomes was extracted.The quantitative characterization parameters of three-dimensional chromosomes were analyzed by artificial image technique.The study found 20 chromosomes in the eggs of ICR female mice,ranging from 0.91 to 15.13 micron.According to the above theory,centromere particles accounted for 30%,near centromere particles 15%,near telomere particles 5% and telomere particles 50%.The study of chromosome morphology in ICR female mice is an important reference for the research and application of ICR female mice.Through the experimental analysis,this paper presents a method to automatically quantify the information of chromosome morphology based on the combination of artificial technology and computer system,which provides an important way for chromosome morphology research and chromosome recognition.