Study On Fast Parallel Measurement Method For Three-dimensional Tracking Of Microspheres Based On CUDA

The three-dimensional tracking of microspheres is an important tool in research of biology,which especially plays a key role in biological single molecule force spectrum measurement.At present,single molecule force spectroscopy can measure the kinetic characteristics of single or a small number of biomolecules,which is inefficient and can not reliably evaluate the difference of biological kinetic characteristics.In recent years,high-precision and real-time parallelization are the two developing directions of the single-molecule force spectrum measurement.The speed and accuracy of threedimensional tracking of the microspheres put forward a very high demand.Through the research of the existing methods,the result is that although there are many kinds of methods for measuring the three-dimensional position of microspheres,few methods that can meet the requirement of fast speed and high precision.Based on centrifugal force microscopy,a novel biomolecule force spectrum parallel testing method,centrifugal force is applied to biomolecule by rotation,and realtime measurement of a large number of biomolecule force spectrum information in a single measurement.The experiment of this technology is urgently needed to realize the high-precision and rapid measurement of the three-dimensional positions of a large number of microspheres.Aiming at the requirement of this method,this paper has carried out the related research to the fast parallel measurement method of threedimensional position of the microsphere,the following contains the main research contents:1.The three-dimensional position tracking system of microspheres was set up,and the measuring principle,experimental device and preparation method of the sample were analyzed.For the centrifugal force microscopy experimental device,biomolecule dynamics experiments were carried out using DNA and other biological samples,and the current instrumentation was evaluated and upgraded.2.A new method for rapid and precise measurement of 3D position of microspheres is presented.The principle of measurement is introduced and the experimental device is set up for verification.The tracking speed of 100 frames per second in the axial direction was achieved,and the resolution of 1nm in the 3D direction of the microsphere is obtained.At the same time,the method is compared with the cross correlation method under the same conditions.3.Based on the CUDA architecture,the parallel measurement method of the three-dimensional position of the microspheres using GPU is studied.The functional architecture and measurement flow of the parallel measurement software are constructed.At the same time,according to the three-dimensional position measurement algorithm proposed in this paper,the core algorithm is programmed to realize some function of the algorithm,which greatly improves the software efficiency.Which is the basis for the parallel measurement of large-volume parallel three-dimensional location of microspheres.