Analysis Of Gene Regulation Mode Based On Chromatin Interaction Data

The three-dimensional spatial structure of chromatin plays an important role in gene transcription regulation.On the one hand,it affects the activity of gene transcriptional regulation region and,on the other hand,regulates the combination of cis-regulatory elements and trans-acting factors in genome.Intron-containing genes and intronless genes have different transcriptional regulation characteristics,and their transcriptional regulatory regions may have different chromatin interactions.Therefore,this topic focuses on the whole genome distribution of chromatin interactions,and analyzes the chromatin interaction pattern of the intron-containing genes and intronless genes in the vicinity of the transcription initiation site.And last,we have studied the relationship between chromatin interaction and gene regulation by using nucleosome position,histone modification,topologically associating domains(TADs),gene enrichment analysis and so on.By analyzing the human chromatin interaction data systematically,we found that the interaction intensity within chromosome was significantly greater than that between chromosomes,and the interaction frequency between small chromosomes(chrl 6-22)was significantly higher than that of larger chromosomes(chrl-9),and the interaction intensity between chromosomes was related to the density of genes.The chromatin interaction pattern of intron-containing genes and intronless genes of human genome in the vicinity of transcription start site(TSS)is completely different.The chromatin interaction trend of multiple exon genes in the range of 2000 bp upstream and downstream of TSS is a "groove" Mode,that is,in the TSS region chromatin interction weak,upstream and downstream distal site chromatin interaction frequency are gradually increased;and a "single.peak" mode was formed around the 600 bp region downstream of TSS of the intronless genes,that is,the intensity of the action in the upper region of TSS is in general,in the region of gene body is the strongest,and then slow decline..In the vicinity of TSS,five and four different chromatin interaction patterns were formed in the intron-containing genes and intronless genes.The average frequency of single exon genes loci is less than the average frequency of multiple exon genes loci,and the average expression level of single exon genes is also significantly lower than that of multiple exon genes.We found that the higher the interaction between the chromatin fragments,the higher the gene expression level,the more regular the distribution of nucleosomes,the higher the level of active histone modification.The stronger the chromatin interaction is,the more likely the gene is to be located inside the TAD and closer to the TAD boundary.