Structure And Functional Analysis Of Inducible Promoter Of The Phyllostachys Edulis MYB Gene

Phyllostachys edulis is abundant bamboo resources with the most widely distributed,the largest planting area and exploitation and utilization of the highest value in china.Phyllostachys edulis usually grows in damp,high temperature,acidic or neutral soil region.Low temperature,drought and dark stress can seriously affect the phyllostachys edulis normal growth.Therefore,the study on its stress resistance mechanism is important significance.This paper studies on resistance gene Pe MTB of phyllostachys edulis,adopting PCR amplification to clone MYB promoter of phyllostachys edulis and bioinformatics analysis,as well as the promoter activity of total length of verification and lack of 5'end of functional analysis.The main results are as follows:1.PeMYB gene promoter was cloned by using PCR amplification.Compared with the sequence of phyllostachys edulis of whole genome,obtained length promoter sequence of 1332 bp of PeMYB gene,gene number was PH01000142.2.Using PLANTCARE software for bioinformatics analysis of PeMYB promoter sequence,the results showed that the PeMYB promoter sequence not only contained the core components of MYB,also contained a lot of light,low temperature,drought,abscisic acid,jasmonic acid,salicylic acid,gibberellin and other abiotic stress related responsive element.3.The PeMYBpro promoter activity was analyzed by constructing the full-length plant expression vector of pcAMBIA1301-PeMYBpro promoter;pcAMBIA1301-PeMYBpro performed transient expression analysis in onion epidermis.GUS gene activity stain showed the PeMYBpro promoter had an inducible promoter activity,and which was strong under low temperature,drought and dark stress induced.4.There were four 5' deletions plant expression vector constructed,which were p CAMBIA1301-PMpro-P1,pCAMBIA1301-PMpro-P2,pCAMBIA1301-PMpro-P3 and p CAMBIA1301-PMpro-P4 respectively.After transient expression analysis,and conducted GUS gene activity staining analysis and quantitative analysis.The results showed that these four fragments had inducible promoter activity,the promoter PeMYBpro between-1332~-1033 not closely related with the drought,low temperature and dark stress promoter three elements.There were a large number of drought stress response elements between-1032~-740.Some components could response to low temperature stress between-450~-141.Between-1032~-740 and-449~-141,there were a lot of promoter element related to light response.