The Research Of Key Factors Of Rapid Development Of The Protonema Of Didymodon Ditrichoides In Loess Region

Biological crust is a common bioactive ground coverage in arid and semi-arid regions,while moss-dominated crusts is the advanced stage of development and succession of biological soil crusts on Loess Region,China,which can grow in drought,poor nutrient,harsh environment habitats,play an important role in promoting soil formation,improving soil physical and chemical propertie and soil resistance to erosion ability and regional ecological environment changes and other aspects.This study use the natural development moss-dominated crust,which dominant specie is Didymodon ditrichoides(Broth.)on Loess Hilly Region as research material,through the indoor culture method,studying the effects of different disinfection method,culture medium and its pH,temperature,light intensity and sugar factors on the growth and differentiation of protonema of the Didymodon ditrichoides(Broth.)by using completely and single-factor experiment design,In order to provide scientific basis for the engineering application of artificial restoration of biological crust.The main results are as follows:(1)Disinfectant and disinfectant time have a significant effect on the survival rate of the cormus of Didymodon ditrichoides,and the disinfectant is the main influencing factor.the suitable method for disinfection of Didymodon ditrichoides is0.1% NaClO disinfection 15 ~ 20 S.(2)The culture medium and its pH had a significant effect on the growth and differentiation of the protonema of Didymodon ditrichoides.Knop and Hoagland culture medium were more suitable for the growth of the protonema of Didymodon ditrichoides,while MS culture medium is not suitable.The pH of the culture medium that is suitable for the growth of the protonema of Didymodon ditrichoides is 7.5.(3)The temperature and light intensity significantly affected the growth and development of the protonema of Didymodon ditrichoides.By completely experiment design found that the temperature was the main influencing factor.At 25 ?,1500 Lx is the most suitable temperature and light intensity for the growth of the protonema of Didymodon ditrichoides.(4)Glucose,sucrose and fructoseall all inhibit the growth and differentiation of the protonema of Didymodon ditrichoides.(5)The best combination for quickly culturing the protonema of Didymodon ditrichoides is: 0.1% NaClO disinfection 15 ~ 20 s + + Hoagland/Knop medium pH = 7.5 + temperature of 25 ? + light intensity of 1500 Lx+ without adding any sugar.