Rat Spermatogenesis Identification And Differentiation Of The Early Stages Of MicroRNA Expression

Nine days postpartum rats were used as the research object to construct the small RNA library of spermatogonium and spermatocytes,which are in the early stages of spermatogenesis.Meanwhile,the miRNAs of spermatogonia and spermatocytes were identified by Solexa high throughput sequencing technique.The target genes of miRNAs were predicted by bioinformatics technique and their functions were analyzed preliminarily.After Solexa sequencing,the two libraries of spermatogonium and spermatocytes were successfully founded including 17639958 and 14759321 raw reads,in which 17179418 and 14182716 were high-quality small RNA sequences,respectively.Bioinformatics analysis of the distinct miRNAsidentified,212 previously known miRNAs and 24 candidate miRNAs were identified in spermatogonium library,205 known miRNAs and 9predicted candidate miRNAs were identified in spermatocytes library.108 known miRNAs were differentially expressed between spermatogonium and spermatocytes libraries,in which 73 miRNAs in spermatogonium library were significantly higher than these in spermatocytes library,35 miRNAs in spermatocytes library were significantly higher than these in spermatogonium library and 126 miRNAs no differences.The miRNAs of the early stage of spermatogenesis in rats were identified by Solexa high-throughput sequencing,and the differences were analyzed.This provides valuable information for the study of spermatogenesis in rats or other animals and provides a basis for further study of the role of miRNAs in rat spermatogenesis and molecular regulation.We performed additional investigation for identifying the potential target m RNAs of 10 highly expressed miRNAs,finally selecting total 87 target genes.These target gene functions are associated with signaling pathways in cancer,apoptosis,sticky spot connection,and ubiquitination.