Preliminary Study Of Mutant Ckrc3-1 And Nbc-1

The level of auxin in plants depends on the balance between its biosynthesis and metabolism,and auxin participates in a variety of physiological and developmental processes in plants,such as plant cell elongation and division,vascular tissue development,plant gravity and phototropism formation,apical dominance,as well as the growth of floral organs.We have already known there are multiple pathways of auxin synthesis in plants.Because the lack of relevant experiment of genes and enzymes,there is little supporting evidence and result of auxin synthesis pathways.N-terminal acetylation is a protein modification in eukaryotes that plays an important role in protein degradation,protein complex formation,subcellular localization and protein folding.We previously isolated an auxin-deficient mutant ckrc3-1,by map-based cloning and whole genomo resequencing,we identified that CKRC3 was AT5G58450,which encodes the auxiliary subunit of B-N-terminal acetyltransferase complex.The catalytic subunit of this complex was encoded by AT1G03150,and we obtained the mutant carrying a T-DNA insertional allele of AT1G03150,it was named nbc-1.This mutant had a low auxin phenotype similar to ckrc3-1,such as the shorter roots and hypocotyls,the absence of roots' geotropism,the phenotype can be restored by auxin and the its insensitivity to cytokinin.Accordingly,we speculate that nbc-1 is also a low auxin mutant.Because NatB may be damaged in both mutants.We hope to explain the auxin synthesis and metabolism process and its control principle by studying the mutants.