Study On Nanomanipulation And Detection Of Elctrical Signals For Single Cells '

The analysis of single cells is an interdisciplinary frontier formed by the disciplines such as analytical chemistry and biomedicine,and it attracts much attention from many researchers because of its wide range of applications.A cell is the basic unit of living things,and it can effectively reflect their functions and statuses.Analyses of cell physiological characteristics are mostly carried out based on the statistical analyses of cell populations,but they do not pay attention to the differences between individual cells,and thus some important information of cells is disregarded.Thus,the analyses of physiological characteristics on single cells are mainly single-means based and short of measurement methods,and the current measurement processes are cumbersome.In this thesis,a robot nano-manipulation system was designed for single living cells,performing a variety of measurements of physiological characteristics and manipulation tasks of cell positioning.The atomic force microscope is an important research tool in nano science and technology with high efficiency,precision and flexibility.It is widely used in the fields such as biomedicine.The robot nanomanipulation platform was designed and built based on atomic force microscopy to complete the development of system control programs,perform the study of physiological characteristics of single living cells,and explore the application functions of the developed system.The large-scale scanning function was developed to overcome the disadvantage of common AFM short scanning range and implement the positioning and imaging of single cells.The mechanics model of cell manipulation was analyzed in detail.A single-probe AFM was used to measure the myocardial force curve and a double-probe AFM was used as the nanotweezers to complete the manipulation of cells.The action potential of cardiomyocytes was measured using a conductive probe as the nanoelectrode.The robot nanomanipulation system designed in this work can be used to complete the measurements of biophysical properties such as morphology,mechanical and electrical signals,and the manipulation task such as positioning in physiological conditions for single living cells.This work provides a new way for the studies of physiological and pathological processes of single cells,the early diagnosis and treatment of major diseases,and the drug screening.