Detection Technique Of Polarization Properties Of Cells

When light transfers in scattering medium,the medium has effects of scattering on the light to some extent.The size and the refractive index of scattered particles can affect the propagation of light.Scattering light of the medium contains different polarization information.In biomedicine,polarized light propagation in the biological tissue has been widely used,which has potential value in the diagnosis of disease and biological tissue imaging.Cell is the smallest unit of living body,so the study of light scattering and optical identification technology aiming at cells will have great scientific value in biomedicine in the future.This thesis presents a polarization microscopic imaging system which can have images of rat hepatocytes and hepatoma cells.Image fusion program are written in MATLAB to dispose the polarization image of cells at different angles.We can acquire the four Stokes' parameters,the degree of polarization,and the angle of polarization images.Then they can be used to express the polarization characteristics of the sample,and infer that the cells are healthy or not.The scattering characteristics of spherical cells,which have different sizes and different refractive index,are analyzed based on Mie scattering theory.And the curve of the relationship between the polarization degree and the scattering angle of scattering light was obtained.We know that the method of finite difference time domain(FDTD)can be used to simulate the model of various particles.In this thesis,we use the FDTD software to simulate the distribution of scattered light intensity of the spherical cells and the ellipsoidal cells,which are under polarized light.This has a good grasp of the study of biological cell polarization imaging,and will be a foundation for further study of the polarization properties of cells.