Based On Acidithiobacillus Ferrooxidans' Proteomics Research,Arsenic And Metal-related Protein Reconstruct Metabolic Pathway

Biometallurgy technology has the advantages of simple operation,economy and environment friendly.The leaching efficiency of microorganisms in biological metallurgy is mainly related to the following factors:(1)A.ferrooxidans reaction ability of iron oxidation reaction and cell metabolism and other factors;(2)Mineral composition of acidophilic thiobacillus ferrous oxide growth is good and oxidation reaction;(3)the temperature and p H range of the dipstick;(4)Acid mine water poisonous ion concentration is more than acidophilic thiobacillus ferrous oxide pain tolerance.In actual industrial production,A.ferrooxidans are often resistant to high concentrations of metal ions in the ore hazards,with the biological leaching process,the leaching environment of heavy metal concentrations continue to rise,and the accumulation of these toxic heavy metals will inhibit the leaching Bacterial activity,which in turn affects leaching efficiency.This study intends to establish a protein data integration system around A.ferrooxidans metabolism ? arsenic and metal-related protein bioinformatics,including the protein-related protein database.Based on the integration of bioinformatics data,the metabolic model of iron metabolism,sulfur metabolism and arsenic metabolism was established for ferrous oxidation system of A.ferrooxidans,sulfur metabolism system and arsenic metabolic system.By studying the mechanism of heavy metal resistance and the resistance mechanism of A.ferrooxidans strain,it is helpful to understand the molecular mechanism of the metal resistance of the strain,which will help us to further improve the conversion efficiency in the industrial application,so as to break through the bottleneck problem of the existing research,Making the application of A.ferrooxidans more operable.In this study,the results of A.ferrooxidans proteomics showed that there were 19 protein spots associated with thiobacillus ferrooxidans in arsenic stress compared with the original strain.Of the expression of more than 3 times,of which 11 protein expression was significantly increased,7 protein expression decreased significantly.11 differential proteins include arsenic resistant protein family transcriptional regulators,ceruloplasmin,cystathionine synthase,heat shock protein,serine protease,quinone oxidoreductase,thiol reductase and thioredoxin.A.ferrooxidans proteomics research strategy,can be used to study the mechanism of cell adaptation in other environmental conditions,such as complex morphological differentiation and development process,extraordinary environmental adaptability and metabolic mechanisms such as physiological and biochemical processes,in order to further promote the development of biological breeding has important reference value and theoretical significance.