Study On The Regulation Of C2C12 Myoblasts Differentiation By Lnc247

A Long non-coding RNAs(lncRNAs)are those over 200 nucleotides in length and do not have a functional open reading frame(ORF).In recent years,many studies show that lncRNA can regulate the gene expression at epigenetic,transcriptional,and post-transcriptional levels through a wide range of mechanisms,and indicating that it plays an important role in the regulation of skeletal muscle development.In this laboratory,we found a new long non-coding RNA Lnc247-an natural antisense RNA of Foxk1 by RNA sequencing.Foxk1 is an important regulatory factor in the development of skeletal muscle,regulating the proliferation and differentiation of myoblasts.Therefore,it is of great significance to study the role of Lnc247 in the regulation of C2C12 myoblast differentiation.To explore the function of Lnc247 in the differentiation of C2C12 myoblasts,we detected Lnc247 and Foxk1 levels by real-time PCR in different mouse tissues,as well as C2C12 myoblasts during myogenesis.The results showed that Lnc247 and Foxk1 was ubiquitously expressed in various tissues,both in the spleen and fat expression were the highest,the heart and muscle were the lowest,Lnc247 and Foxk1 was downregulated during cell differentiation.When upregulated or downregulated the expression of Lnc247 in C2C12 myoblasts,we used real-time PCR and Western blotting to detect the expression of myogenic marker gene MHC and myogenic regulatory factor MyoG,The myotubule formation was detected by cell immunouorescent staining.After expression of upregulated Lnc247,the results indicated that the fuction of cell differentiation was significantly enhanced.The above results show that Lnc247 can promote the differentiation of C2C12 myoblasts.These findings provided a theoretical basis for elucidating LncRNA regulation of skeletal muscle differentiation.