Distribution And Migration Of CD133~+ Neural Stem Cells In Embryo Mouse Brain

Neural stem cells(NSCs)are stem cells with the ability of self-renewal and multiple differentiation potential.Under certain circumstances,NSCs are able to differentiate into neurons,oligodendrocytes and astrocytes,participating in functional recovery of neural system,which is known as neurogenesis.Neurogenesis mainly occurs in the central nervous system of mouse embryonic period,meanwhile it can be regulated via a variety of signaling pathways.Recent studies have shown that when central nervous system are injuried,NSCs will be migrate to the damaged area,the new neurons replace the missing cells and the surrounding neurons to establish signaling pathways.Volkan Coskun et al.(2007)demonstrated that C D133 ~+ neural stem cells can produce new neurons using genetic pedigree tracer methods,and new neurons can migrate to olfactory bulbs.CD133~+ cells are compared to GFAP~+ B cells more generally represent the NSCs in adult brain.CD133~+ neural stem cells migrate to olfactory bulb through rostral migratory stream(RMS),which differentiate into type A cells and intermediate neurons.This is also demonstrates that C D133~+ cells have NSCs characteristics.In 2005,Luo et al.Confirmed the presence of CD133~+ neural stem cells in SVZ of adult mouse.In this paper we research the distribution and migratory stream of CD133 ~+ neural stem cells in different neuroanatomical regions of embryo mouse brain,which is based on the good results of CD133~+ neural stem cell research.Experimental results show that the distributions of NSCs are significantly different,the distribution o f dorsal was more than ventral side.Zs Green cells are mainly distributed in SVZ,the third ventricle,the fourth ventricle,aqueduct,hippocampus.CD133~+ neural stem cells are mainly distributed in the SVZ,the third ventricle,the fourth ventricle,aqueduct area.In addition,with the growth and development of embryo mouse,a large number of CD133 ~+ neural stem cells migrated from SVZ to the surrounding ganglion,corpus callosum and cerebral cortex,and C D133 ~+ neural stem cells were differentiated into other types of cells.For the third ventricle of embryo mouse brain,we find C D133 ~+ neural stem cells on the ependymal membrane of the third ventricle migrate to the surrounding hypothalamus region,and CD133 ~+ neural stem cells are differentiated into other types of cells.Finally,we compared the distribution of CD133~+ neural stem cells in embryo mouse brain and two weeks old mouse,the distribution of CD133~+ neural stem cells in embryo mouse brain was found different compared to two weeks old mice in SVZ.The distribution of CD133~+ neural stem cells in the 2-week-old mouse are distributed throughout the fourth ventricle ependymal region,while the embryo mous are mainly distributed in the dorsal region of the 4V ependyma.The exploration of NSCs distrib ution and migration will not only greatly promote the development of neuroscience,but also provide a new treatment for central nervous system-related diseases.Therefore,the reseach of neural stem cell distribution and migration have important scientific significance and clinical application value.