Cloning And Functional Analysis Of TaCBL1 Gene In Wheat

In the natural environment,plants are often subject to multiple stress stimuli.These stimuli can be sensed by plants and produce different forms of response through a variety of signal transduction pathways.Calcium(Ca2+)signaling is a very important way to transduce a large number of stimuli or signals in plants.Ca2+ is involved in the regulation of various types of calcium decoders to mediate signal transduction in plants.In these decoders,calcineurin B-like proteins(CBL)and the kinase-specific proteins of the serine-threonine kinase kinase(CIPK)that interact specifically with CBL Complexes and play a very important role in the transduction of these signals.These two gene families are involved in a variety of stimulus-response coupled signal network pathways.Although the CBL-CIPK network has proven to play a key role in plant development and various environmental stress responses,little is known about the function of wheat.In order to understand the role of wheat CBL1,the TaCBLl gene was first cloned and the bioinformatics analysis was carried out.The expression pattern of TaCBLl gene under different abiotic stress was analyzed by qRT-PCR.The TaCBLl gene was transferred into tobacco by Agrobacterium tumefaciens,and its response to abiotic stress was observed.The morphological indexes,physiological and biochemical indexes,Revealing the effect of overexpression of TaCBLl on the growth and development of tobacco under abiotic stress.The main results are as follows:1.The results showed that TaCBL1 gene was significantly different from PEG-6000(20%),high salt(200 mM NaCI),low temperature(4?)and ABA(100 ?M),and the effects of different abiotic stress,the expression level of TaCBL1 gene in TaCBLl gene was significantly decreased,and then the TaCBL1 gene was significantly decreased after 3h,The expression level of TaCBL1 was low at 12 hours,and the expression level of TaCBL1 was lower in low temperature(4?),The expression le-vel of TaCBLl gene in leaves and roots was decreased gradually,and the expression of TaCBL1 gene in leaves and roots was decreased gradually,and the expression of TaCBLl gene in leaves and roots was decreased gradually.But the expression of the gene in the roots decreased faster.2.The TaCBLl gene was constructed on the pCAMBIA1300 vector.The localization of the TaCBL1 gene in the tobacco protoplasts showed that the TaCBL1 protein was localized on the cell membrane,indicating that TaCBL1 was active on the plasma membrane.3.The TaCBLl gene was transferred into tobacco using Agrobacterium tumefaciens to observe its phenotype under different abiotic stresses.It is interesting to show that it is sensitive to salt,which is consistent with previous studies on AtCBL1 in Arabidopsis thaliana Salt resistance is different,of course,we understand that predecessors in the study of poplar PeCBL1 gene also has a similar phenotype.4.In order to further explore why TaCBLl was transferred into tobacco,we selected the leaf and root tissues of transgenic tobacco under different concentrations of salt,and made physiological and biochemical analysis and sodium and potassium ion content.The results showed that Tobacco leaves and roots of K+/Na+,wild type than transgenic tobacco is higher.