Cloning And Function Analysis Of Caleosin Gene From Ricinus Communis

Biodiesel is a renewable bioenergy. It can be used as a substitute for fossil fuel. Biodiesel plays an important role in alleviating the energy crisis and reducing environmental pollution. Through the gene engineering method regulating the synthesis of fatty acid in plant can improve oil content in plants, so as to provide more raw materials of biodiesel production. The oil body is the main oil storage organelles in plant cells. Caleosin as an important component of the oil body, it is closely related to oil body formation, oil body quantity, oil body structure stability and the accumulation of the fatty acid in plant cells. Tobacco has the advantages of rapid propagation, large biomass, and mature genetic transformation system, Improving the oil content in tobacco leaves by means of gene regulation can be used to extract oils and fats to produce biodiesel, For this purpose, the following experiments were done:1. The Caleosin gene was cloned from Ricinus communis, Extraction of total RNA from different tissues and different developmental stages of castor oil, the semi quantitative expression of the gene was analyzed.The results show that the the expression of Caleosin gene in leaves, stems, roots,20d,30d,40d after flowering seed was detected.The amount of expression in seed is higher than that in other tissues. And mainly concentrated in the late stage of seed development (oil accumulation period),It shows that the expression of Caleosin gene and the process of oil accumulation are synchronous.2. The cloned castor Caleosin gene was connected to the plant expression vector pCAMBIA 1303, The recombinant plant expression vector pCAMBIA-RcClo containing castor Caleosin gene was constructed. The recombinant expression vector was transformed into tobacco by Agrobacterium mediated leaf disk transformation, Transgenic tobacco plants were obtained.3. Determination of fatty acid content in leaves of transgenic tobacco plants, the results showed that the fatty acid content of transgenic tobacco leaves increased by 8.1% compared with the wild type tobacco leaves.