| This paper focused on the Clematis fruticosa Turcz of Daqingshan Mountains,we collected their rhizosphere soil and radicle,arbuscular mycorrhizal fungi spores were divided and identificated from them,and the mycorrhizal infection rates were measured.The AMF vaccination experiments were carried out in the laboratory,and the drought stress was proceeded when the mycorrhiza was formed.Then the physiological and biochemical indexes of Clematis fruticosa Turcz under normal water and drought stress,high-throughput sequencing was carried out about the Transcriptome of Clematis fruticosa Turcz under water stress.It was Explored that how the drought resistances of Clematis fruticosa were affected by inoculation of AMF at the physiological and molecular levels.The purpose of this study was to understand AMF Community Composition in rhizosphere soil and radicle of Clematis fruticosa Turcz,and to investigate the effect of AMF on the growth and drought tolerance of Clematis fruticosa Turcz in arid environment.The main research results were as follows:1.The results showed that Clematis fruticosa Turcz was a typical arbuscular mycorrhizal plant,and its mycorrhizal infection rate was over 90%.2.10 genera and 45 species of AMF spores were divided from Clematis fruticosa Turcz rhizosphere soil,among which the importance value of Funneliformis mosseae?Rhizophagus intraradices and Acaulaspora mellea were more than 50%.It was meaned that they were dominant species.The importance value of G.glomerulatum,R.aggregatus,G.versiform were more than average value,so they belonged to common species.3.The results of one-way ANOVA analysis of variance showed that the abundance,Shannon-Weiner index,Simpson index and evenness of AMF community diversity index were significantly changed with the seasons,but slightly with the altitude.The results which came from the analysis of AMF diversity index and the environmental factors RDA showed that the soil factors had an effect on AMF species,but the correlation of the different soil factors and AMF species had differences.PerMANOVA analysis results showed that the seasons had a bigger impact on AMF community composition in rhizosphere soil of Clematis fruticosa Turcz than the altitude.4.The inoculation R.intraradices of F%,a%,A% were significantly(P< 0.05)greater than inoculation of A.mellea and F.mosseae under drought stress condition.There was no significant difference between the A.mellea and F.mosseae groups(P>0.05).The m% of R.i treatment was significantly higher than A.mellea,which had no significant difference with F.mosseae.There was no significant difference about M% among the three inoculation treatments(P>0.05).5.Inoculated AMF remarkablely decreased MDA content in leaves of Clematis fruticosa Turcz,Significantly increased the activity of SOD,POD and CAT in protective enzymes(P<0.05)under drought stress condition.The content of Pro in mycorrhizal seedlings was significantly lower than that in the untreated control group(CK2).6.Compared with the untreated control group(CK2),the inoculation treatment had significantly improved(P<0.05)the total N content and absorption of P elements under drought stress.7.Transcriptome sequencing was performed on the Clematis fruticosa Turcz in the untreated and inoculated R.intraradices treatment group.The unigenes sequence was compared with the public databases Swiss-Prot,Nr,Pfam,KEGG,KOG,and GO which based on the sequence similarity by BLAST search.Finally,differential expression genes(DEGs)were analyzed.A total of 2691 DEGs were obtained from DEGs which were screened by |log2fold change |?1 and P? 0.001.Compared with the untreated treatment,1108 DEGs were up-regulated and 1853 DEGs were down-regulated during inoculation. |
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