The Generation Of Arabidopsis Thaliana Suc4p/psal Double Mutant And The Function Analysis Of SUC4

Sucrose transporters(SUTs) are ubiquitous membrane proteins involved in the cellular transmembrane transport of sucrose in plants and play a major role upon growth and development. Our previous research had shown that the occurrence of functional interaction between sucrose transporter At SUC4 and photosystem I subunit At Psa L, and the site was located in chloroplasts. To further understand the relationship between sugar transporters and photosynthetic mechanism, in this study, we investigated the interaction between At SUC4 and At Psa L by using reverse genetics strategy.Through generation of Arabidopsis thaliana suc4p/psal double mutants, we harvested four homozygous double mutants. Observing phenotypes show that there are significant differences between atsuc4p/atpsal-1,atpsal-1/atsuc4 p,atpsal-2/atsuc4 p and wild type,three double mutants, compared with wild type, were low and small,and showed curly and reduced rosette leaves, increased and close-packed fruits, while atsuc4 p /atpsal-2 kept the same with wild type on the phenotype. Analysis of Real-Time PCR experiments of the reciprocal cross of atsuc4 p and atpsal show that the transcription of SUC4 in double mutant were down-regulated little, and PSAL were unchanged compared with its atpsal parents.Assays that detect SUTs transport sucrose or not through esculin uptake condition were proved that Jatropha curcas sucrose transporter Jc SUT1 is able to transport sucrose.besides, we found that multiple yeast strains are compatible with this assay, including invertase wild-type strains. So we could find a novel assay for sucrose transporter activity in the future.