Analysis Of Typical Phase Microscopic Imaging System Of Biological Cells And It’s Common Optical Path Improvement

As the basic composition unit of organism and life activity, cell’s morphological structure is complicated, and function is various, and they can reflect the various base of organisms accurately and timely. The recognition and analysis of cell is becoming more and more attention in many fields of science, especially in the biological medicine, the research of pathogenesis of the disease, diagnosis of disease and assessment of therapeutic effects, which are all dependent on the detection and study of biological cells. In recent years, a variety of digital quantitative phase microscopy technology is developing rapidly. For its rapid and can be quantitative, non-invasive,quantitative huge advantage in terms of biological cell measurement, it has been widely used. Optical path of the stability, accuracy and information extraction of presentation is one of the most important factors to affect optical biological cell detection. Therefore, improving the biological cell phase imaging technology in order to obtain high stability, high accuracy of the research is a very meaningful work.In this paper, the cell detection technology of optical path for system analysis,and studies its light path structure and main use of optics, found that a good experimental results do not necessarily need a good experiment platform and the high cost of optical devices. Based on the universality of application of Mach-Zehnder interference light path, the traditional phase microscopy imaging under Mach-Zehnder optical path dynamic simulation experiment was carried out, and set up two kinds of structure design of Mach-Zehnder phase microscopy optical path, conducted a comparative study, the results of simulation and experiment showed that the results obtained by the simple experimental structure are not necessarily bad.Based on the requirements of phase microscopy imaging technology for low cost,high stability, and application of typical phase microscopy optical path combined with knowledge of geometrical optics, we design a free phase microscopy method of microscope objectives in common optical path. The method just need simple opticaldevice, such as lens, reflector, the experimental operation is simple. And adopt the form of common optical path, avoid the material such as noise and different way with the result of the experiment error. According to the design of microscope objectives in common optical path phase microscopy, structured the verification experimental light path, obtained the interference pattern. And compared with the experimental results of which based on the Mach-Zehnder optical path experiment, verify the feasibility and high efficiency of the light path. in reconstruction of biological cell morphological structure, the experimental results have very good application value. And the experimental results have provide a good explore ideas for more in-depth study of the late train of thought.