Partial Epigenetic Factor Analysis In The Development Of AC3 Knock Mouse MOE

Adenylate cyclase plays an important role in mouse olfacory generated.AC3 knockout mice showed obesity, anosmia, learning and memory deficits, the main olfactory epidermal tissue?MOE? with the growth performance of mice MOE thin, loosely structured tissue cells, dendritic growth retardation and other phenomena.Using gene chips to analysis AC3 knockout mice MOE found that the gene expression in AC3 deletion mouse MOE was significant changed, including a number of epigenetic factors such as Tet3, BAF53 b. Epigenetic refers to stably heritable phenotype resulting from changes in a chromosome without alterations in the DNA sequence.Epigenetic cell development process plays an important role in cell development.The study found that Tet3 overexpression disrupts olfactory receptor expressionand there's a physiologically significant role for BAF53 b in Activity-Dependent Dendrite Growth.So how BAF53 b and Tet3 changes in protein levels after AC3 knockout?Tet3 is the most highlyexpressed Tet family member in the MOE,which can oxidize5 m C to 5-hydroxymethylcytosine?5hm C?.5hm C is enrichedin neurons where it may contribute to gene regulation and cellularidentity.AC3 knockout have any effect on 5m C and 5hm C?H3K27me3 has an imortant role in neural differentitation,expression Tet3 acoomanied with Tet3 gene promoter regions H3K27me3 level decline,whether AC3 deletions affect H3K27me3 expression?With the development of mouse MOE,howthe expression level of5 m C,5hm C,Tet3,H3K27me3 and BAF53 b changes?These questions are yet to be resolved.In order to solve the above problems,our esperiment taked 7day,30 day and 90 day as different developmental stages of AC3mutation(AC3^-/-) mice and their wild-type(AC3^+/+) littermate mice MOE organization as materials.Usingimmunofluorescence techniquesto stain DNA modification 5m C / 5hm C, DNA modification regulator TET3, histone lysine modification H3K27me3, histone structural adjustment factor BAF53 b.It was found that as AC3 knocked out,within the same age,the level of 5m C is not significantchanged, but the expression of Tet3 is upregulated, the level of 5hm C occurs down,the expression of H3K27me3 and BAF53 b is reduced. Along with the growth of the age, the expression of 5hm C, BAF53 b and Tet3 performance is reduced,theexpression of H3K27me3 showed increases, the level of 5m C expression is not significantchanged.The above results give the fact that after AC3 deletion, not only caused part of the expression of epigenetic factor differential expression,and cause some level of epigenetic marks in the chromosome changed.We hypothesized that,as AC3 knockout c AMP signaling pathway is blocked, the stimulation from odor molecules is insensitive to immature olfactory neurns, leading to reduced expression BAF53 b,and then impaired development of dendrites. The reduction of H3K27me3 lead to the increase expression of Tet3, Tet3 increased expression can oxidize5 m C to 5-hydroxymethylcytosine?5hm C? and further to form5-formylcytosine?5f C? and 5-carboxylcytosine?5ca C?, and then enter the DNA methylation process. Resulting inthe down-regulated expression of 5hm C,whichlead to differential expressions of genes?...