Detection Of Glucose, Peroxidase With Electrochemical And Luminescence Methods

The detection of bio-molecules such as glucose or enzyme is important in the areas include forensic analysis,healthy & medicine.This thesis give a primary study of the electrochemical response of glucose on nanoporous gold,and the chemical luminescence of peroxidase in neutrophils.Several chapters are comprised in this thesis:The methods of electrochemical analysis and chemi-luminescence analysis are summarized.Preparation of the nanporous gold electrode.The electrocatalytic oxidation of glucose at nanoprous gold?NPG?electrodes was studied.Glucose can be electrooxidized at NPG in both neutral solution?phosphate buffer,pH=7.4?and alkaline solution?0.1 mol/L NaOH?.In neutral solutions,the NPG electrodes can be easily poisoned by chloride ions?Cl-?.However,the NPG electrode can resist the poison of Cl-in alkaline solutions.A linear scan voltamemtry for determination of glucose of physiological concentrations was developed.The interference of some substance in physiological environment such as L-ascorbic acid?AA?,uric acid?UA?and 4-acetamidophenol?AP?was also investigated.An imaging analysis method based on enhanced chemiluminescence?CL?detection was developed and applied to obtain chemical information of single cells.The system of enzyme-peroxidase?PO?catalyzing the luminol/H₂O₂/p-iodophenol reaction was adopted in this paper.PO information from 10 neutrophils immobilized on a glass substrate were imaging by CL simultaneously within 5s,which proves that a sensitive and high throughput method based on the CL imaging technique had been developed.