Detection Of Glucose, Peroxidase With Electrochemical And Luminescence Methods

The detection of bio-molecules such as glucose or enzyme is important in the areas include forensic analysis,healthy & medicine.This thesis give a primary study of the electrochemical response of glucose on nanoporous gold,and the chemical luminescence of peroxidase in neutrophils.Several chapters are comprised in this thesis:The methods of electrochemical analysis and chemi-luminescence analysis are summarized.Preparation of the nanporous gold electrode.The electrocatalytic oxidation of glucose at nanoprous gold（NPG）electrodes was studied.Glucose can be electrooxidized at NPG in both neutral solution（phosphate buffer,pH=7.4）and alkaline solution（0.1 mol/L NaOH）.In neutral solutions,the NPG electrodes can be easily poisoned by chloride ions（Cl-）.However,the NPG electrode can resist the poison of Cl-in alkaline solutions.A linear scan voltamemtry for determination of glucose of physiological concentrations was developed.The interference of some substance in physiological environment such as L-ascorbic acid（AA）,uric acid（UA）and 4-acetamidophenol（AP）was also investigated.An imaging analysis method based on enhanced chemiluminescence（CL）detection was developed and applied to obtain chemical information of single cells.The system of enzyme-peroxidase（PO）catalyzing the luminol/H₂O₂/p-iodophenol reaction was adopted in this paper.PO information from 10 neutrophils immobilized on a glass substrate were imaging by CL simultaneously within 5s,which proves that a sensitive and high throughput method based on the CL imaging technique had been developed.