The Development And Testing Of Protein Folding Experiment System Based On Microfluidic Mixer

Protein is a linear sequence of amino acids by peptide bond mutual connection, but there is no physiological function of linear polypeptide chain, only when it has physiological function after formed a unique three-dimensional structure. The new linear polypeptide chain constitute a three-dimensional structure of a particular process is called protein folding. However accurately and correctly folded into a particular structure is a complex process, the temperature, ph value, chemical will appear under the influence of misfolding, may lead to loss and lower protein biological activity, and even the happening of the disease.Protein folding refers to the process of protein to obtain the functional structureand conformation. That is how the protein folds into a three-dimensional structure withbiological functions from a simple peptide chain with non-biological functions. At present, the protein folding can be studied experimentally through a variety of experimentalmethods, mainly X-ray crystallography, NMR spectroscopy, electron microscopy 3D reconstruction, scanning tunneling microscopy and UV fluorescence technology. Here in this article, we through ultraviolet fluorescence technology to get information on protein folding. Based on this ultrafast microfluidic mixer, the process of protein folding kinetics is studied by optical detection to get relevant information about protein folding.Ultrafast microfluidic mixer is a kind of focusing microfluidic mixer, the mixer can overcome Dean Vortex which is generated by high flow rates, that is to say it can achieve flow rates at high Reynolds number. This microfluidic focusing mixer uses hydrodynamic focusing technology to focus the protein sample into a jet. The jet is only 100 nanometers wide. Its mixing time is short and it has a little sample consumption. Using the pressure to drive protein sample to flow and they can mix rapidly within microfluidic chip of the container.266 nm ultraviolet excitation light source excitation test samples (N-acetyl-L tryptophan amine (NATA)) fluorescence and optical information gathered in photoelectric detector, and by the reaction of nano displacement platform along the microfluidic chip scanning optical information channel movement, and will scan position into time, time-resolved fluorescence information, according to the fluorescence intensity information collected can get early data of protein folding. Through MATLAB programming processing experimental data to acquire the information of protein folding.This paper designed a set of experimental system based on Ultrafast microfluidic mixer, adopted LABVIEW programming to write the graphical user interface, using LABVIEW voice running into a program designed to a virtual instrument, the operator can be directly on the control panel for intuitive, including photoelectric detection drive, pneumatic drive,scanning drive. On the pneumatic drive, the operator can directly input pressure values on the control panel on the control panel and through the sensor can show the actual pressure value size; On the photoelectric detection driver, the operator can be real-time continuous running the program the fluctuation magnitude of intensity values; On the Scan driver, we installed in the objective lens 100 microns objective drive, so that to 100* 100 mm X-Z direction the scanning of the area to find fluorescence is focused on the strongest point location, and observation of the chip jet in 500 microns channel respectively for 6 times 10* 100 microns area of X-Y scanning direction. In this paper, the main work is to build a set of detecting fluorescence intensity of the light path system, the velocity of the liquid in the microfluidic chip control and the scanning system of chip control, and the whole system was tested and ensure the completeness of the system.