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The Life Tables Of Gryllodes Sigillatus And Preliminary Isolation And Identification Of Its Intestinal Microorganism

Posted on:2017-06-03Degree:MasterType:Thesis
Country:ChinaCandidate:H Z WangFull Text:PDF
GTID:2310330485957248Subject:Plant protection
Abstract/Summary:PDF Full Text Request
Gryllodes sigillatus, belonging to Gryllodes, Ensifera, Nematocera, Grylloidea, Gryllidae, Gryllodes, commonly known as horse stove cricket, cooking chicken is a kind of important renewable biological resources. It has much value in the side of eating,medicinal and feed.Its utilization as resources in the country has not been large-scale expansion at present.In the exeriment, exploring the parameters of the experimental population, isolating and identifing the intestinal microorganism of experimental populations in the gut of 8 kinds of dominant bacteria group preliminarily. It can provide scientific theoretical basis and guidance for Gryllodes sigillatus resources development and utilization.1?The age-stage life table of Gryllodes sigillatus Experimental populationIn the laboratory, feeding Chinese cricket population under the condition of 30 ?, 9L:15D, RH60%.Then recording each instar development time, survival, mortality, fecundity and other datas about life history, Analysising all the datas by using the software of age-sex life table(Chi and Liu, 1985; Chi, 1988). The Gryllodes sigillatus eggs can last about 15 days, and the average larval developmental period is about 63.2d,About 60% larvae can develop into adult, of which the proportion of female adults was 60%, and the proportion of male adult was 40%. Male life expectancy can last 147.08 d, the female is 145.67 d. The fecundity of single female(In place of newly hatched larvae) is 274. The daily highest fecundity was 75. The intrinsic rate of increase(r) is 0.046871d-1. The finite rate of increase(lambda) is 1.047987d-1. The net reproductive rate(R0) is 83.78 generations/individual. The population generation the average period was 94.476 d(T).2?Intestinal microorganism of the Gryllodes sigillatus experimental populationThrough the separation of Gryllodes sigillatus adult intestinal bacteria from the environment purification, there are 8 strains of bacteria. The bacteria morphology, culture characters, dyeing and physiological and biochemical reactions were studied systematically. The results show that the eight bacterial strains were as follows : Serratia ?Microbacterium ? Klebsiella ? Salmonella ? Staphylococcus ? Escherichia ? luyvera ?Bacillus.
Keywords/Search Tags:Gryllodes sigillatus, Twosex life table, Intestinal microorganism
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